Construct: ORF TRCN0000467698
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005349.3_s317c1
- Derived from:
- ccsbBroadEn_14606
- DNA Barcode:
- TGGTAAGTTTTACCCAGAACCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DGKA (1606)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467698
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_001345.5 | 99.9% | 99.8% | 1612C>T |
| 2 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_001351033.1 | 99.9% | 99.8% | 1612C>T |
| 3 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_001351034.1 | 99.9% | 99.8% | 1612C>T |
| 4 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_201444.2 | 99.9% | 99.8% | 1612C>T |
| 5 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_201445.1 | 99.9% | 99.8% | 1612C>T |
| 6 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_201554.1 | 99.9% | 99.8% | 1612C>T |
| 7 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_011537993.2 | 99.9% | 99.8% | 1612C>T |
| 8 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_001351035.1 | 95% | 94.9% | 1_114del;1726C>T |
| 9 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_017018908.1 | 89.7% | 89.6% | 1_114del;513_650del;1864C>T |
| 10 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_017018901.2 | 86.1% | 86% | 400_753del;1966C>T |
| 11 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_017018902.1 | 86.1% | 86% | 400_753del;1966C>T |
| 12 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_017018903.1 | 86.1% | 86% | 400_753del;1966C>T |
| 13 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_017018904.1 | 86.1% | 86% | 400_753del;1966C>T |
| 14 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_017018905.1 | 86.1% | 86% | 400_753del;1966C>T |
| 15 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_017018906.2 | 86.1% | 86% | 400_753del;1966C>T |
| 16 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_017018907.1 | 86.1% | 86% | 400_753del;1966C>T |
| 17 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_005268689.2 | 83.9% | 81.2% | (many diffs) |
| 18 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_011537995.2 | 83.9% | 81.2% | (many diffs) |
| 19 | human | 1606 | DGKA | diacylglycerol kinase alpha | XM_017018900.1 | 82.4% | 82.3% | 1_114del;514_867del;2080C>T |
| 20 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_001351036.1 | 80.7% | 80.6% | 0_1ins423;1189C>T |
| 21 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_001351037.1 | 80.7% | 80.6% | 0_1ins423;1189C>T |
| 22 | human | 1606 | DGKA | diacylglycerol kinase alpha | NR_147026.1 | 79.7% | (many diffs) | |
| 23 | human | 1606 | DGKA | diacylglycerol kinase alpha | XR_002957294.1 | 78.9% | (many diffs) | |
| 24 | human | 1606 | DGKA | diacylglycerol kinase alpha | XR_002957296.1 | 75.2% | (many diffs) | |
| 25 | human | 1606 | DGKA | diacylglycerol kinase alpha | NR_147027.1 | 72.1% | (many diffs) | |
| 26 | human | 1606 | DGKA | diacylglycerol kinase alpha | XR_001748602.1 | 70.7% | (many diffs) | |
| 27 | human | 1606 | DGKA | diacylglycerol kinase alpha | XR_002957293.1 | 70% | (many diffs) | |
| 28 | human | 1606 | DGKA | diacylglycerol kinase alpha | XR_002957295.1 | 58.6% | (many diffs) | |
| 29 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_001351038.1 | 51.7% | 51.7% | 0_1ins1062;550C>T |
| 30 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_001351039.1 | 51.7% | 51.7% | 0_1ins1062;550C>T |
| 31 | human | 1606 | DGKA | diacylglycerol kinase alpha | NM_001351040.1 | 51.7% | 51.7% | 0_1ins1062;550C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2271
- ORF length:
- 2205
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caaggagagg ggcctaataa gccccagtga ttttgcccag ctgcaaaaat 121 acatggaata ctccaccaaa aaggtcagtg atgtcctaaa gctcttcgag gatggcgaga 181 tggctaaata tgtccaagga gatgccattg ggtacgaggg attccagcaa ttcctgaaaa 241 tctatctcga agtggataat gttcccagac acctaagcct ggcactgttt caatcctttg 301 agactggtca ctgcttaaat gagacaaatg tgacaaaaga tgtggtgtgt ctcaatgatg 361 tttcctgcta cttttccctt ctggagggtg gtcggccaga agacaagtta gaattcacct 421 tcaagctgta cgacacggac agaaatggga tcctggacag ctcagaagtg gacaaaatta 481 tcctacagat gatgcgagtg gctgaatacc tggattggga tgtgtctgag ctgaggccga 541 ttcttcagga gatgatgaaa gagattgact atgatggcag tggctctgtc tctcaagctg 601 agtgggtccg ggctggggcc accaccgtgc cactgctagt gctgctgggt ctggagatga 661 ctctgaagga cgacggacag cacatgtgga ggcccaagag gttccccaga ccagtctact 721 gcaatctgtg cgagtcaagc attggtcttg gcaaacaggg actgagctgt aacctctgta 781 agtacactgt tcacgaccag tgtgccatga aagccctgcc ttgtgaagtc agcacctatg 841 ccaagtctcg gaaggacatt ggtgtccaat cacatgtgtg ggtgcgagga ggctgtgagt 901 ccgggcgctg cgaccgctgt cagaaaaaga tccggatcta ccacagtctg accgggctgc 961 attgtgtatg gtgccaccta gagatccacg atgactgcct gcaagcggtg ggccatgagt 1021 gtgactgtgg gctgctccgg gatcacatcc tgcctccatc ttccatctat cccagtgtcc 1081 tggcctctgg accggatcgt aaaaatagca aaacaagcca gaagaccatg gatgatttaa 1141 atttgagcac ctctgaggct ctgcggattg accctgttcc taacacccac ccacttctcg 1201 tctttgtcaa tcctaagagt ggcgggaagc aggggcaaag ggtgctctgg aagttccagt 1261 atatattaaa ccctcgacag gtgttcaacc tcctaaagga tggtcctgag atagggctcc 1321 gattattcaa ggatgttcct gatagccgga ttttggtgtg tggtggagac ggcacagtag 1381 gctggattct agagaccatt gacaaagcta acttgccagt tttgcctcct gttgctgtgt 1441 tgcccctggg tactggaaat gatctggctc gatgcctaag atggggagga ggttatgaag 1501 gacagaatct ggcaaagatc ctcaaggatt tagagatgag taaagtggta catatggatc 1561 gatggtctgt ggaggtgata cctcaacaaa ctgaagaaaa aagtgaccca gtcccctttc 1621 aaatcatcaa taactacttc tctattggcg tggatgcctc tattgctcat cgattctaca 1681 tcatgcgaga gaaatatccg gagaagttca acagcagaat gaagaacaag ctatggtact 1741 tcgaatttgc cacatctgaa tccatcttct caacatgcaa aaagctggag gagtctttga 1801 cagttgagat ctgtgggaaa ccgctggatc tgagcaacct gtccctagaa ggcatcgcag 1861 tgctaaacat ccctagcatg catggtggct ccaacctctg gggtgatacc aggagaccCC 1921 ATGGGGATAT CTATGGGATC AACCAGGCCT TAGGTGCTAC AGCTAAAGTC ATCACCGACC 1981 CTGATATCCT GAAAACCTGT GTACCAGACC TAAGTGACAA GAGACTGGAA GTGGTTGGGC 2041 TGGAGGGTGC AATTGAGATG GGCCAAATCT ATACCAAGCT CAAGAATGCT GGACGTCGGC 2101 TGGCCAAGTG CTCTGAGATC ACCTTCCACA CCACAAAAAC CCTTCCCATG CAAATTGACG 2161 GAGAACCCTG GATGCAGACG CCCTGTACAA TCAAGATCAC CCACAAGAAC CAGATGCCCA 2221 TGCTCATGGG CCCACCCCCC CGCTCCACCA ATTTCTTTGG CTTCTTGAGC TACCCAACTT 2281 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2341 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2401 CTTGTGGAAA GGACGATGGT AAGTTTTACC CAGAACCTTT ACGCGTTAAG TCgacaatca 2461 acctctggat tacaaaattt gtgaaagatt