Construct: ORF TRCN0000467704
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003218.1_s317c1
- Derived from:
- ccsbBroadEn_11596
- DNA Barcode:
- ACGTATTATTGTGGTACCCCGTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRDM5 (11107)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467704
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_005262708.3 | 31.5% | 28.4% | (many diffs) |
| 2 | human | 11107 | PRDM5 | PR/SET domain 5 | NM_001300824.1 | 21.8% | 19.8% | (many diffs) |
| 3 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_017007669.1 | 20.5% | 18.6% | (many diffs) |
| 4 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_017007670.1 | 20.5% | 18.6% | (many diffs) |
| 5 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_011531567.2 | 20.1% | 18.2% | (many diffs) |
| 6 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_011531568.2 | 20.1% | 18.2% | (many diffs) |
| 7 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_011531569.3 | 20.1% | 18.2% | (many diffs) |
| 8 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_011531565.2 | 18.5% | 16.8% | (many diffs) |
| 9 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_011531566.3 | 18.5% | 16.8% | (many diffs) |
| 10 | human | 11107 | PRDM5 | PR/SET domain 5 | NM_001300823.1 | 18.2% | 16.6% | (many diffs) |
| 11 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_011531564.2 | 18% | 16.4% | (many diffs) |
| 12 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_011531563.2 | 17.9% | 16.3% | (many diffs) |
| 13 | human | 11107 | PRDM5 | PR/SET domain 5 | NM_018699.3 | 17.3% | 15.8% | (many diffs) |
| 14 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_011531562.2 | 17% | 15.5% | (many diffs) |
| 15 | human | 11107 | PRDM5 | PR/SET domain 5 | XR_938680.2 | 16.6% | (many diffs) | |
| 16 | human | 11107 | PRDM5 | PR/SET domain 5 | XR_938678.1 | 16.1% | (many diffs) | |
| 17 | human | 11107 | PRDM5 | PR/SET domain 5 | XR_938679.1 | 15.5% | (many diffs) | |
| 18 | human | 11107 | PRDM5 | PR/SET domain 5 | XR_938677.3 | 15.4% | (many diffs) | |
| 19 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_017007668.2 | 12.7% | 10.7% | (many diffs) |
| 20 | mouse | 70779 | Prdm5 | PR domain containing 5 | XM_006506602.3 | 28.1% | 29.5% | (many diffs) |
| 21 | mouse | 70779 | Prdm5 | PR domain containing 5 | NM_029549.1 | 23.5% | 23.6% | (many diffs) |
| 22 | mouse | 70779 | Prdm5 | PR domain containing 5 | XM_011241464.2 | 20% | 19.4% | (many diffs) |
| 23 | mouse | 70779 | Prdm5 | PR domain containing 5 | NM_027547.2 | 16.7% | 16.2% | (many diffs) |
| 24 | mouse | 70779 | Prdm5 | PR domain containing 5 | XM_006506604.3 | 15.9% | 15.4% | (many diffs) |
| 25 | mouse | 70779 | Prdm5 | PR domain containing 5 | XR_377493.3 | 14.8% | (many diffs) | |
| 26 | mouse | 70779 | Prdm5 | PR domain containing 5 | XR_377492.3 | 13.6% | (many diffs) | |
| 27 | mouse | 70779 | Prdm5 | PR domain containing 5 | XR_377491.3 | 10.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 423
- ORF length:
- 333
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCTGGGCAT GTACGTGCCG GACAGGTTCT 121 CCCTGAAGTC CTCCCGGGTT CAGGACGGCA TGGGGCTCTA CACGGCCCGC AGAGTGCGAA 181 AGGGTGAAAA GTTCGGACCC TTTGCTGGAG AGAAGAGAAT GCCTGAAGAC TTGGATGAAA 241 ATATGGATTA CAGGTTGATG TGGGAGGTTC GTGGGAGTAA GGGAGAAGTT TTGTACATTT 301 TGGATGCTAC CAACCCACGG CACTCCAACT GGCTTCGCTT CGTTCATGAG GCACCATCTC 361 AGGAGCAGAA GAACTTGGCT GCCATTCAAG ACAAGAATTT GGGACCCGCC GAATGGCGAG 421 GCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 481 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 541 GGCTTTATAT ATCTTGTGGA AAGGACGAAC GTATTATTGT GGTACCCCGT CTACGCGTTA 601 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt