Construct: ORF TRCN0000467709

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008908.1_s317c1
Derived from:
ccsbBroadEn_12624
DNA Barcode:
ATCTACCTATCTCATTCGATGCCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ARHGAP28 (79822)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467709
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79822 ARHGAP28 Rho GTPase activating prote... NM_001010000.3 68.2% 68.2% (many diffs)
2 human 79822 ARHGAP28 Rho GTPase activating prote... NM_001366230.1 53.3% 53.3% (many diffs)
3 human 79822 ARHGAP28 Rho GTPase activating prote... XM_005258146.2 48.5% 48.5% (many diffs)
4 human 79822 ARHGAP28 Rho GTPase activating prote... NM_001366231.1 48.2% 45.6% (many diffs)
5 mouse 268970 Arhgap28 Rho GTPase activating prote... XM_006524341.3 48.8% 52.2% (many diffs)
6 mouse 268970 Arhgap28 Rho GTPase activating prote... XM_006524342.3 48.8% 52.2% (many diffs)
7 mouse 268970 Arhgap28 Rho GTPase activating prote... XM_006524343.3 48.8% 52.2% (many diffs)
8 mouse 268970 Arhgap28 Rho GTPase activating prote... NM_001347410.1 48.7% 52.1% (many diffs)
9 mouse 268970 Arhgap28 Rho GTPase activating prote... NM_172964.4 45.4% 48.7% (many diffs)
10 mouse 268970 Arhgap28 Rho GTPase activating prote... XM_017317488.1 42.1% 45% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1236
ORF length:
1170
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa gaaaatccgc catctctctc tgattgaatt gactgccttt tttgatgcct
121ttggaattca actgaaaagg aacaaaacag agaaagtaaa aggacgagac aatgggattt
181ttggagttcc acttacagtc ctcctggacg gtgaccgaaa gaaagaccct ggagtgaaag
241ttcccctggt attacaaaaa ttttttgaga aagttgagga atcaggtctg gaatctgaag
301gaatttttcg actttcagga tgtactgcta aagtcaagca ataccgtgaa gaacttgatg
361ccaagtttaa tgctgataaa tttaaatggg acaaaatgtg ccatagagaa gctgcagtaa
421tgttgaaagc gtttttcaga gaactaccca cctctctctt ccctgtggaa tatatacctg
481ccttcatcag tctaatggaa agagggcctc acgtcaaagt acagtttcaa gccttacacc
541tcatggtcat ggcgctgcct gatgccaaca gagatgcagc tcaggccctc atgacattct
601tcaataaagt gattgccaat gaatcaaaaa accgaatgag tctgtggaac atttctacag
661tgatggcacc gaaccttttc ttcagtagaa gcaaacactc tgattatgaa gaattactgt
721tagcaaacac tgcggcccac atcatccgcc taatgcttaa gtaccagaag attttgtgga
781aggttccgtc tttcttaatc actcaagtaa gaaGAATGAA TGAAGCCACG ATGCTATTGA
841AGAAGCAGCT CCCAAGTGTC AGGAAGCTGC TCAGGAGGAA GACCCTCGAG CGGGAGACTG
901CAAGCCCCAA GACTTCAAAG GTACTGCAAA AATCACCCTC GGCAAGACGA ATGTCTGACG
961TGCCGGAAGG AGTCATACGG GTCCATGCTC CACTTCTCTC CAAGGTGTCC ATGGCCATTC
1021AACTCAACAA TCAAACCAAA GCCAAAGACA TATTGGCAAA ATTTCAATAT GAAAACAGTC
1081ATGGTTCATC AGAATGTATT AAGATTCAGA ACCAAAGGTT ATATGAAATT GGAGGAAATA
1141TAGGAGAGCA TTGCTTGGAT CCAGATGCTT ATATATTGGA TGTATATCGT ATAAATCCTC
1201AAGCAGAATG GGTGATTAAA CCCCAACCAA GTTCTTACCC AACTTTCTTG TACAAAGTGG
1261TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1321CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1381AATCTACCTA TCTCATTCGA TGCCGACGCG TTAAGTCgac aatcaacctc tggattacaa
1441aatttgtgaa agatt

Download FASTA (ORF) (Full)