Construct: ORF TRCN0000467709
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008908.1_s317c1
- Derived from:
- ccsbBroadEn_12624
- DNA Barcode:
- ATCTACCTATCTCATTCGATGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGAP28 (79822)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467709
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79822 | ARHGAP28 | Rho GTPase activating prote... | NM_001010000.3 | 68.2% | 68.2% | (many diffs) |
| 2 | human | 79822 | ARHGAP28 | Rho GTPase activating prote... | NM_001366230.1 | 53.3% | 53.3% | (many diffs) |
| 3 | human | 79822 | ARHGAP28 | Rho GTPase activating prote... | XM_005258146.2 | 48.5% | 48.5% | (many diffs) |
| 4 | human | 79822 | ARHGAP28 | Rho GTPase activating prote... | NM_001366231.1 | 48.2% | 45.6% | (many diffs) |
| 5 | mouse | 268970 | Arhgap28 | Rho GTPase activating prote... | XM_006524341.3 | 48.8% | 52.2% | (many diffs) |
| 6 | mouse | 268970 | Arhgap28 | Rho GTPase activating prote... | XM_006524342.3 | 48.8% | 52.2% | (many diffs) |
| 7 | mouse | 268970 | Arhgap28 | Rho GTPase activating prote... | XM_006524343.3 | 48.8% | 52.2% | (many diffs) |
| 8 | mouse | 268970 | Arhgap28 | Rho GTPase activating prote... | NM_001347410.1 | 48.7% | 52.1% | (many diffs) |
| 9 | mouse | 268970 | Arhgap28 | Rho GTPase activating prote... | NM_172964.4 | 45.4% | 48.7% | (many diffs) |
| 10 | mouse | 268970 | Arhgap28 | Rho GTPase activating prote... | XM_017317488.1 | 42.1% | 45% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1236
- ORF length:
- 1170
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gaaaatccgc catctctctc tgattgaatt gactgccttt tttgatgcct 121 ttggaattca actgaaaagg aacaaaacag agaaagtaaa aggacgagac aatgggattt 181 ttggagttcc acttacagtc ctcctggacg gtgaccgaaa gaaagaccct ggagtgaaag 241 ttcccctggt attacaaaaa ttttttgaga aagttgagga atcaggtctg gaatctgaag 301 gaatttttcg actttcagga tgtactgcta aagtcaagca ataccgtgaa gaacttgatg 361 ccaagtttaa tgctgataaa tttaaatggg acaaaatgtg ccatagagaa gctgcagtaa 421 tgttgaaagc gtttttcaga gaactaccca cctctctctt ccctgtggaa tatatacctg 481 ccttcatcag tctaatggaa agagggcctc acgtcaaagt acagtttcaa gccttacacc 541 tcatggtcat ggcgctgcct gatgccaaca gagatgcagc tcaggccctc atgacattct 601 tcaataaagt gattgccaat gaatcaaaaa accgaatgag tctgtggaac atttctacag 661 tgatggcacc gaaccttttc ttcagtagaa gcaaacactc tgattatgaa gaattactgt 721 tagcaaacac tgcggcccac atcatccgcc taatgcttaa gtaccagaag attttgtgga 781 aggttccgtc tttcttaatc actcaagtaa gaaGAATGAA TGAAGCCACG ATGCTATTGA 841 AGAAGCAGCT CCCAAGTGTC AGGAAGCTGC TCAGGAGGAA GACCCTCGAG CGGGAGACTG 901 CAAGCCCCAA GACTTCAAAG GTACTGCAAA AATCACCCTC GGCAAGACGA ATGTCTGACG 961 TGCCGGAAGG AGTCATACGG GTCCATGCTC CACTTCTCTC CAAGGTGTCC ATGGCCATTC 1021 AACTCAACAA TCAAACCAAA GCCAAAGACA TATTGGCAAA ATTTCAATAT GAAAACAGTC 1081 ATGGTTCATC AGAATGTATT AAGATTCAGA ACCAAAGGTT ATATGAAATT GGAGGAAATA 1141 TAGGAGAGCA TTGCTTGGAT CCAGATGCTT ATATATTGGA TGTATATCGT ATAAATCCTC 1201 AAGCAGAATG GGTGATTAAA CCCCAACCAA GTTCTTACCC AACTTTCTTG TACAAAGTGG 1261 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1321 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1381 AATCTACCTA TCTCATTCGA TGCCGACGCG TTAAGTCgac aatcaacctc tggattacaa 1441 aatttgtgaa agatt