Transcript: Mouse NM_001347410.1

Mus musculus Rho GTPase activating protein 28 (Arhgap28), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Arhgap28 (268970)
Length:
5161
CDS:
90..2126

Additional Resources:

NCBI RefSeq record:
NM_001347410.1
NBCI Gene record:
Arhgap28 (268970)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001347410.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000418222 AGCCCTAGACAAGTCTTTAAA pLKO_005 2119 CDS 100% 15.000 12.000 N Arhgap28 n/a
2 TRCN0000178866 CCTAGTGATTCTTGTGAGCAT pLKO.1 498 CDS 100% 2.640 2.112 N Arhgap28 n/a
3 TRCN0000423484 ATGGTTCATCTGAACATATTA pLKO_005 1981 CDS 100% 15.000 10.500 N Arhgap28 n/a
4 TRCN0000429834 ATGGTTCCTGCCCTGTATTAT pLKO_005 2152 3UTR 100% 15.000 10.500 N Arhgap28 n/a
5 TRCN0000418889 TTAGATCCTGATGCATATATA pLKO_005 2055 CDS 100% 15.000 10.500 N Arhgap28 n/a
6 TRCN0000426977 ATGGAAGAAGTCGGACATTAA pLKO_005 863 CDS 100% 13.200 9.240 N Arhgap28 n/a
7 TRCN0000441080 TTGAGGCTATCCCGGATATTG pLKO_005 685 CDS 100% 13.200 9.240 N Arhgap28 n/a
8 TRCN0000432572 AGTACTCAGATGCTACGTTAT pLKO_005 2573 3UTR 100% 10.800 7.560 N Arhgap28 n/a
9 TRCN0000179210 GCGGGAAATTGAGAGTATCAA pLKO.1 182 CDS 100% 5.625 3.938 N Arhgap28 n/a
10 TRCN0000183452 GAAGGTTCCATCTTTCTTGAT pLKO.1 1679 CDS 100% 4.950 3.465 N Arhgap28 n/a
11 TRCN0000195897 CCTGATGCTGAAGTACCAGAA pLKO.1 1649 CDS 100% 4.050 2.835 N Arhgap28 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001347410.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12624 pDONR223 100% 48.7% 52.1% None (many diffs) n/a
2 ccsbBroad304_12624 pLX_304 0% 48.7% 52.1% V5 (many diffs) n/a
3 TRCN0000467709 ATCTACCTATCTCATTCGATGCCG pLX_317 36.3% 48.7% 52.1% V5 (many diffs) n/a
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