Construct: ORF TRCN0000467718
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001498.1_s317c1
- Derived from:
- ccsbBroadEn_11169
- DNA Barcode:
- CTCCGACATGGTCATCACCCGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADAM17 (6868)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467718
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6868 | ADAM17 | ADAM metallopeptidase domai... | NM_003183.6 | 25.3% | 25% | (many diffs) |
| 2 | human | 6868 | ADAM17 | ADAM metallopeptidase domai... | XM_011510375.3 | 21.6% | 20.9% | (many diffs) |
| 3 | mouse | 11491 | Adam17 | a disintegrin and metallope... | NM_009615.6 | 21.7% | 20.9% | (many diffs) |
| 4 | mouse | 11491 | Adam17 | a disintegrin and metallope... | NM_001277266.1 | 21.2% | 20.4% | (many diffs) |
| 5 | mouse | 11491 | Adam17 | a disintegrin and metallope... | NR_120376.1 | 12% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 693
- ORF length:
- 627
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaaa 61 ttggcatgag gcagtctctc ctattcctga ccagcgtggt tcctttcgtg ctggcgccgc 121 gacctccgga tgacccgggc ttcggccccc accagagact cgagaagctt gattctttgc 181 tctcagacta cgatattctc tctttatcta atatccagca gcattcggta agaaaaagag 241 atctacagac ttcaacacat GTAGAAACAC TACTAACTTT TTCAGCTTTG AAAAGGCATT 301 TTAAATTATA CCTGACATCA AGTACTGAAC GTTTTTCACA AAATTTCAAG GTCGTGGTGG 361 TGGATGGTAA AAACGAAAGC GAGTACACTG TAAAATGGCA GGACTTCTTC ACTGGACACG 421 TGGTTGGTGA GCCTGACTCT AGGGTTCTAG CCCACATAAG AGATGATGAT GTTATAATCA 481 GAATCAACAC AGATGGGGCC GAATATAACA TAGAGCCACT TTGGAGATTT GTTAATGATA 541 CCAAAGACAA AAGAATGTTA GTTTATAAAT CTGAAGATAT CAAGAATGTT TCACGTTTGC 601 AGTCTCCAAA AGTGTGTGGT TATTTAAAAG TGGATAATGA AGAGTTGCTC CCAAAAGGGT 661 TAGTAGACAG AGAACCACCT GAAGGTAAGA CTTACCCAAC TTTCTTGTAC AAAGTGGTTG 721 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 781 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACT 841 CCGACATGGT CATCACCCGG CAACGCGTTA AGTCgacaat caacctctgg attacaaaat 901 ttgtgaaaga tt