Construct: ORF TRCN0000467800
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016746.1_s317c1
- Derived from:
- ccsbBroadEn_12916
- DNA Barcode:
- CCATCTTGTACCCAATCACTGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SSH2 (85464)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467800
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85464 | SSH2 | slingshot protein phosphata... | NM_001282131.2 | 88.1% | 88.1% | 1_72del |
| 2 | human | 85464 | SSH2 | slingshot protein phosphata... | NM_001282130.2 | 87.1% | 82.5% | (many diffs) |
| 3 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_017025255.2 | 75.8% | 67.7% | (many diffs) |
| 4 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_017025254.2 | 34% | 29.7% | (many diffs) |
| 5 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_017025253.2 | 30.9% | 27% | (many diffs) |
| 6 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_005258059.2 | 12% | 11.5% | (many diffs) |
| 7 | human | 85464 | SSH2 | slingshot protein phosphata... | NM_033389.3 | 11.6% | 10.4% | (many diffs) |
| 8 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_011525402.2 | 11.5% | 10.1% | (many diffs) |
| 9 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_006722149.4 | 11.4% | 10.4% | (many diffs) |
| 10 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_011525404.2 | 11.4% | 10.5% | (many diffs) |
| 11 | human | 85464 | SSH2 | slingshot protein phosphata... | NM_001282129.2 | 11.3% | 10% | (many diffs) |
| 12 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_005258058.3 | 11.3% | 10% | (many diffs) |
| 13 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_017025251.2 | 11.3% | 9.9% | (many diffs) |
| 14 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_011525405.2 | 11.1% | 10.4% | (many diffs) |
| 15 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_011525406.2 | 8% | 6.6% | (many diffs) |
| 16 | human | 85464 | SSH2 | slingshot protein phosphata... | XM_011525407.1 | 8% | 6.6% | (many diffs) |
| 17 | mouse | 237860 | Ssh2 | slingshot homolog 2 (Drosop... | XM_006533229.3 | 11.1% | 11.4% | (many diffs) |
| 18 | mouse | 237860 | Ssh2 | slingshot homolog 2 (Drosop... | NM_001291190.1 | 11% | 11.3% | (many diffs) |
| 19 | mouse | 237860 | Ssh2 | slingshot homolog 2 (Drosop... | NM_177710.4 | 10.8% | 10.3% | (many diffs) |
| 20 | mouse | 237860 | Ssh2 | slingshot homolog 2 (Drosop... | XM_006533227.3 | 10.7% | 9.9% | (many diffs) |
| 21 | mouse | 237860 | Ssh2 | slingshot homolog 2 (Drosop... | XM_006533228.3 | 10.6% | 9.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 600
- ORF length:
- 534
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tccttacttc tccgccaacg cggtcatctc gcagaacgcc atcaaccagc 121 tcatcagcga gagctttcta actgtcaaag gtgctgccct ttttctacca cggggaaatg 181 gctcatccac accaagaatc agccacagac ggaacaagca tgcaggcgat ctccaacagc 241 atctccaagc aatgttcaTT TTACTCCGCC CAGAAGACAA CATCAGGCTG GCTGTAAGAC 301 TGGAAAGTAC TTACCAGAAT CGAACACGCT ATATGGTAGT GGTTTCAACT AATGGTAGAC 361 AAGACACTGA AGAAAGCATC GTCCTAGGAA TGGATTTCTC CTCTAATGAC AGTAGCACTT 421 GTACCATGGG CTTAGTTTTG CCTCTCTGGA GCGACACGCT AATTCATTTG GATGGTGATG 481 GTGGGTTCAG TGTATCGACG GATAACAGAG TTCACATATT CAAACCTGTA TCTGTGCAGG 541 CAATGTGGGT TGACAGGGAT TCAAGGAACA AACACTGTGA TGTACTATTG GTGGAAGAAT 601 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 661 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 721 TTTATATATC TTGTGGAAAG GACGACCATC TTGTACCCAA TCACTGTTAA CGCGTTAAGT 781 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt