Construct: ORF TRCN0000467801
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015095.1_s317c1
- Derived from:
- ccsbBroadEn_05906
- DNA Barcode:
- ACTTGGTTAAGTTCGTTCAGCCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BTC (685)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467801
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 685 | BTC | betacellulin | NM_001729.4 | 99.8% | 99.4% | 370T>A |
| 2 | human | 685 | BTC | betacellulin | XM_011532211.1 | 99.8% | 99.4% | 370T>A |
| 3 | human | 685 | BTC | betacellulin | NM_001316963.2 | 72.4% | 72.4% | 281_282ins147 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 600
- ORF length:
- 534
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ccgggccgcc cggtgcagcg gcgccagctc cctgccactg ctcctggccc 121 ttgccctggg tctagtgatc cttcactgtg tggtggcaga tgggaattcc accagaagtc 181 ctgaaactaa tggcctcctc tgtggagacc ctGAGGAAAA CTGTGCAGCT ACCACCACAC 241 AATCAAAGCG GAAAGGCCAC TTCTCTAGGT GCCCCAAGCA ATACAAGCAT TACTGCATCA 301 AAGGGAGATG CCGCTTCGTG GTGGCCGAGC AGACGCCCTC CTGTGTCTGT GATGAAGGCT 361 ACATTGGAGC AAGGTGTGAG AGAGTTGACT TGTTTTACCT AAGAGGAGAC AGAGGACAGA 421 TTCTGGTGAT TTGTATGATA GCAGTTATGG TAGTTTTTAT TATTTTGGTC ATCGGTGTCT 481 GCACATGCTG TCACCCTCTT CGGAAACGTC GTAAAAGAAA GAAGAAAGAA GAAGAAATGG 541 AAACTCTGGG TAAAGATATA ACTCCTATCA ATGAAGATAT TGAAGAGACA AATATTGCTT 601 TCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 661 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 721 TTTATATATC TTGTGGAAAG GACGAACTTG GTTAAGTTCG TTCAGCCCGA CGCGTTAAGT 781 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt