Construct: ORF TRCN0000467811
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006827.1_s317c1
- Derived from:
- ccsbBroadEn_05948
- DNA Barcode:
- CAGAGTGTGCTTTTGCATGCGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCND2 (894)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467811
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 894 | CCND2 | cyclin D2 | NM_001759.4 | 99.8% | 100% | 570C>G |
| 2 | mouse | 12444 | Ccnd2 | cyclin D2 | NM_009829.3 | 88.7% | 91.7% | (many diffs) |
| 3 | mouse | 12444 | Ccnd2 | cyclin D2 | XM_006505459.2 | 88.7% | 91.7% | (many diffs) |
| 4 | mouse | 12444 | Ccnd2 | cyclin D2 | XM_006505460.1 | 88.7% | 91.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 933
- ORF length:
- 867
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gctgctgtgc cacgaggtgg acccggtccg cagggccgtg cgggaccgca 121 acctgctccg agacgaccgc gtcctgcaga acctgctcac catcgaggag cgctaccttc 181 cgcagtgctc ctacttcaag tgcgtgcaga aggacatcca accctacatg cgcagaatgg 241 tggccacctg gatgctggag gtctgtgagg aacagaagtg cgaagaagag gtcttccctc 301 tggccatgaa ttacctggac cgtttcttgg ctggggtccc gactccgaag tcccatctgc 361 aactcctggg tgctgtctgc atgttcctgg cctccaaact caaagagacc agcccgctga 421 ccgcggagaa gctgtgcatt tacaccgaca actccatcaa gcctcaggag ctgctggagt 481 gggaactggt ggtgctgggg aagttgaagt ggaacctggc agctgtcact cctcatgact 541 tcattgagca caTCTTGCGC AAGCTGCCCC AGCAGCGGGA GAAGCTGTCT CTGATCCGCA 601 AGCATGCTCA GACCTTCATT GCTCTGTGTG CCACGGACTT TAAGTTTGCC ATGTACCCAC 661 CGTCGATGAT CGCAACTGGA AGTGTGGGAG CAGCCATCTG TGGGCTCCAG CAGGATGAGG 721 AAGTGAGCTC GCTCACTTGT GATGCCCTGA CTGAGCTGCT GGCTAAGATC ACCAACACAG 781 ACGTGGATTG TCTCAAAGCT TGCCAGGAGC AGATTGAGGC GGTGCTCCTC AATAGCCTGC 841 AGCAGTACCG TCAGGACCAA CGTGACGGAT CCAAGTCGGA GGATGAACTG GACCAAGCCA 901 GCACCCCTAC AGACGTGCGG GATATCGACC TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 961 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1021 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 1081 GAGTGTGCTT TTGCATGCGC GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1141 ttgtgaaaga tt