Construct: ORF TRCN0000467822
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008088.1_s317c1
- Derived from:
- ccsbBroadEn_10362
- DNA Barcode:
- CGCCTCGGACTAACTGACTCCGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS10P7 (376693)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467822
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6204 | RPS10 | ribosomal protein S10 | NM_001014.5 | 43% | 36.3% | (many diffs) |
| 2 | human | 6204 | RPS10 | ribosomal protein S10 | NM_001203245.3 | 43% | 36.3% | (many diffs) |
| 3 | human | 6204 | RPS10 | ribosomal protein S10 | NM_001204091.2 | 43% | 36.3% | (many diffs) |
| 4 | human | 376693 | RPS10P7 | ribosomal protein S10 pseud... | NR_026667.1 | 30.9% | 1_152del;369_699del | |
| 5 | human | 100529239 | RPS10-NUDT3 | RPS10-NUDT3 readthrough | NM_001202470.2 | 24.3% | 20.6% | (many diffs) |
| 6 | mouse | 67097 | Rps10 | ribosomal protein S10 | NM_025963.3 | 40.6% | 36.3% | (many diffs) |
| 7 | mouse | 67097 | Rps10 | ribosomal protein S10 | XM_011246579.2 | 30.1% | 27% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 282
- ORF length:
- 216
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGTT GATGCCTAAG AAGAACTGGA TTGCCATTTA TGAACTCCTT TTTAAGGAGG 121 GAGTCATGGT GGCCAAGAAG GATGTCCACA TGCCTAAGCA CCCGGAGCTG GCAGACAAGA 181 ATGCGCCCAA CCTTCGTGTC ATGAAGGCCA TGCAGTCTCT CAAGTCCCGA GGCTACGTGA 241 AGGAAGTTTG CCTGGAGACA TTTCTACTGG TACCTTACCA ATGCCCAACT TTCTTGTACA 301 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 361 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 421 AGGACGACGC CTCGGACTAA CTGACTCCGA TACGCGTTAA GTCgacaatc aacctctgga 481 ttacaaaatt tgtgaaagat t