Construct: ORF TRCN0000467833
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015068.1_s317c1
- Derived from:
- ccsbBroadEn_07862
- DNA Barcode:
- TAGGACATTCCAATACTGCTACGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASTN2 (23245)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467833
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23245 | ASTN2 | astrotactin 2 | NM_198186.3 | 99.9% | 99.7% | 1262C>T |
| 2 | human | 23245 | ASTN2 | astrotactin 2 | NM_198188.2 | 86.9% | 86.4% | (many diffs) |
| 3 | human | 23245 | ASTN2 | astrotactin 2 | NM_198187.3 | 86.5% | 86.4% | 0_1ins147;1115C>T;1173_1206delinsG |
| 4 | human | 23245 | ASTN2 | astrotactin 2 | NM_001184734.1 | 84.8% | 84.3% | (many diffs) |
| 5 | human | 23245 | ASTN2 | astrotactin 2 | NM_001184735.1 | 42.3% | 39.6% | (many diffs) |
| 6 | human | 23245 | ASTN2 | astrotactin 2 | NM_014010.5 | 33.3% | 31.5% | (many diffs) |
| 7 | human | 23245 | ASTN2 | astrotactin 2 | NM_001365069.1 | 32.1% | 30.4% | (many diffs) |
| 8 | human | 23245 | ASTN2 | astrotactin 2 | NM_001365068.1 | 32% | 30.3% | (many diffs) |
| 9 | mouse | 56079 | Astn2 | astrotactin 2 | NM_019514.3 | 30.1% | 30.7% | (many diffs) |
| 10 | mouse | 56079 | Astn2 | astrotactin 2 | XM_006538104.3 | 29.1% | 29.6% | (many diffs) |
| 11 | mouse | 56079 | Astn2 | astrotactin 2 | NM_207109.2 | 29% | 29.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1386
- ORF length:
- 1320
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggaatgaa cacattgctc tgtaaaggaa tgttttgcct actcagctgg gaggccgaca 121 gcagaggcag acttggggag tacactctgc agcccctgtc cctgcagact gaagagacca 181 cagagctggg cagcaagaag gagctcaagt ccatgccctt catcacctac ctctcaggtt 241 tgctgacagc ccagatgctg tcagatgacc agctcatttc aggtgtggag attcgctgtg 301 aggagaaggg gcgctgtcca tctacctgtc acctttgccg ccggccaggc aaggagcagc 361 tgagccccac accagtgctg ctggaaatca accgtgtggt gccactttat accctcatcc 421 aagacaatgg cacaaaggag gccttcaaga gtgcactgat gagttcctac tggtgctcag 481 ggaaagggga tgtgatcgat gactggtgca ggtgtgacct cagcgccttt gatgccaatg 541 ggctccccaa ctgcagcccc cttctgcagc cggtgctgcg gctgtcccca acagtggagc 601 cctccagtac tgtggtctcc ttggagtggg tggatgttca gccagctatt gggaccaagg 661 tctccgacta tattctgcag cataagaaag tggatgaata cacagacact gacctgtaca 721 caggagaatt cctgagtttt gctgatgact tactctctgg cctgggcaca tcttgtgtag 781 cagctggtcg aagccatgga gaggtccctg aagtcagtat ctactcagtc atcttcaagt 841 gtctggagcc cgacggtctc tacaagttca ctctgtatgc tgtggataca cgagggaggc 901 actcagagct aagcacggtg acccTGAGGA CGGCCTGTCC ACTGGTAGAT GACAACAAGG 961 CAGAAGAAAT AGCTGACAAG ATCTACAATC TGTACAATGG GTACACAAGT GGAAAGGAGC 1021 AGCAGATGGC CTACAACACA CTGATGGAGG TCTCAGCCTC GATGCTGTTC CGAGTCCAGC 1081 ACCACTACAA CTCTCACTAT GAAAAGTTTG GCGACTTCGT CTGGAGAAGT GAGGATGAGC 1141 TGGGGCCCAG GAAGGCCCAC CTGATTCTAC GGCGACTGGA GAGGGTGAGT AGCCACTGCT 1201 CCAGCCTCCT GCGGAGTGCC TACATCCAGA GCCGCGTGGA AACAGTGCCC TATCTTTTCT 1261 GCCGCAGCGA GGAGGTCCGG CCTGCAGGCA TGGTGTGGTA TAGCATCCTC AAGGACACCA 1321 AAATCATGTG TGAGGAGAAG ATGGTGTCAA TGGCCCGAAA CACGTACGGG GAGTCCAAGG 1381 GCCGGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1441 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1501 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATAGGACATT CCAATACTGC TACGCACGCG 1561 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt