Construct: ORF TRCN0000467838
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014102.1_s317c1
- Derived from:
- ccsbBroadEn_04252
- DNA Barcode:
- TCCCTATCTAGTACACCTACTGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP1LC3B (81631)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467838
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81631 | MAP1LC3B | microtubule associated prot... | NM_022818.5 | 100% | 100% | |
| 2 | human | 643246 | MAP1LC3B2 | microtubule associated prot... | NM_001085481.3 | 99.4% | 99.2% | 30G>C;338G>A |
| 3 | mouse | 67443 | Map1lc3b | microtubule-associated prot... | NM_026160.4 | 85.1% | 95.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 465
- ORF length:
- 375
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCCGTCGGA GAAGACCTTC AAGCAGCGCC 121 GCACCTTCGA ACAAAGAGTA GAAGATGTCC GACTTATTCG AGAGCAGCAT CCAACCAAAA 181 TCCCGGTGAT AATAGAACGA TACAAGGGTG AGAAGCAGCT TCCTGTTCTG GATAAAACAA 241 AGTTCCTTGT ACCTGACCAT GTCAACATGA GTGAGCTCAT CAAGATAATT AGAAGGCGCT 301 TACAGCTCAA TGCTAATCAG GCCTTCTTCC TGTTGGTGAA CGGACACAGC ATGGTCAGCG 361 TCTCCACACC AATCTCAGAG GTGTATGAGA GTGAGAAAGA TGAAGATGGA TTCCTGTACA 421 TGGTCTATGC CTCCCAGGAG ACGTTCGGGA TGAAATTGTC AGTGTACCCA ACTTTCTTGT 481 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 541 AGTAATGAAC TAGTCCGTAA CTTGAAAGCA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 601 GAAAGGACGA TCCCTATCTA GTACACCTAC TGAAACGCGT TAAGTCgaca atcaacctct 661 ggattacaaa atttgtgaaa gatt