Construct: ORF TRCN0000467850
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009884.1_s317c1
- Derived from:
- ccsbBroadEn_11836
- DNA Barcode:
- GACATATCATTCCATTGCGATCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJC2 (27000)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467850
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27000 | DNAJC2 | DnaJ heat shock protein fam... | NM_001129887.2 | 25.9% | 24.5% | 430_431ins47;455_1704del |
| 2 | human | 27000 | DNAJC2 | DnaJ heat shock protein fam... | NM_014377.3 | 23.7% | 22.5% | 430_431ins47;455_1863del |
| 3 | human | 27000 | DNAJC2 | DnaJ heat shock protein fam... | NM_001362667.1 | 12.1% | 10.9% | 0_1ins222;208_209ins47;233_1641del |
| 4 | mouse | 22791 | Dnajc2 | DnaJ heat shock protein fam... | NM_009584.4 | 23.8% | 21.5% | (many diffs) |
| 5 | mouse | 22791 | Dnajc2 | DnaJ heat shock protein fam... | XM_006535679.3 | 12.9% | 10.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 567
- ORF length:
- 501
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gcttctgcca agcgccgcgg acggccgggg caccgccatc acccacgctc 121 tgacctctgc ctctacacTC TGTCAAGTTG AACCTGTGGG AAGATGGTTT GAAGCTTTTG 181 TTAAGAGGAG AAACAGAAAT GCTTCTGCCT CTTTTCAGGA ACTGGAGGAT AAGAAAGAGT 241 TATCCGAGGA ATCAGAAGAT GAAGAATTGC AGTTGGAAGA GTTTCCCATG CTGAAAACAC 301 TTGATCCCAA AGACTGGAAG AACCAAGATC ATTATGCAGT TCTTGGACTT GGCCATGTGA 361 GATACAAGGC TACACAGAGA CAGATCAAAG CAGCTCATAA AGCAATGGTT TTAAAACATC 421 ACCCAGACAA ACGGAAAGCA GCTGGTGAAC CAATAAAAGA AGGAGATAAT GACTACTTCA 481 CTTGCATAAC TAAAGGATCC CCAGGTGATT CTTATCAGAA GCTCATCCTA CAGTTAATTC 541 ATCTTATGAA ATGTTATCTG ATCCAGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 601 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 661 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGACATATC 721 ATTCCATTGC GATCGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 781 aagatt