Construct: ORF TRCN0000467853
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003408.1_s317c1
- Derived from:
- ccsbBroadEn_06664
- DNA Barcode:
- CTGACCAAGCAAATGACCAGGCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OAS2 (4939)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467853
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4939 | OAS2 | 2'-5'-oligoadenylate synthe... | NM_002535.3 | 99.9% | 100% | 753A>T |
| 2 | human | 4939 | OAS2 | 2'-5'-oligoadenylate synthe... | NM_016817.3 | 95.4% | 94.9% | (many diffs) |
| 3 | human | 4939 | OAS2 | 2'-5'-oligoadenylate synthe... | XR_001748725.2 | 40.1% | (many diffs) | |
| 4 | human | 4939 | OAS2 | 2'-5'-oligoadenylate synthe... | NM_001032731.2 | 24.3% | 22.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2127
- ORF length:
- 2061
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg aaatggggag tcccagctgt cctcggtgcc tgctcagaag ctgggttggt 121 ttatccagga atacctgaag ccctacgaag aatgtcagac actgatcgac gagatggtga 181 acaccatctg tgacgtcctg caggaacccg aacagttccc cctggtgcag ggagtggcca 241 taggtggctc ctatggacgg aaaacagtct taagaggcaa ctccgatggt acccttgtcc 301 tcttcttcag tgacttaaaa caattccagg atcagaagag aagccaacgt gacatcctcg 361 ataaaactgg ggataagctg aagttctgtc tgttcacgaa gtggttgaaa aacaatttcg 421 agatccagaa gtcccttgat gggttcacca tccaggtgtt cacaaaaaat cagagaatct 481 ctttcgaggt gctggccgcc ttcaacgctc tgagcttaaa tgataatccc agcccctgga 541 tctatcgaga gctcaaaaga tccttggata agacaaatgc cagtcctggt gagtttgcag 601 tctgcttcac tgaactccag cagaagtttt ttgacaaccg tcctggaaaa ctaaaggatt 661 tgatcctctt gataaagcac tggcatcaac agtgccagaa aaaaatcaag gatttaccct 721 cgctgtctcc gtatgccctg gagctgctta cggtgtatgc ctgggaacag gggtgcagaa 781 aagacaactt tgacattgct gaaggcgtca gaaccgttct ggagctgatc aaatgccagg 841 agaagctgtg tatctattgg atggtcaact acaactttga agatgagacc atcaggaaca 901 tcctgctgca ccagctccaa tcagcgaggc cagtaatctt ggatccagtt gacccaacca 961 ataatgtgag tggagataaa atatgctggc aatggctgaa aaaagaagct caaacctggt 1021 tgacttctcc caacctggat aatgagttac ctgcaccatc ttggaatgtt ctgcctgcac 1081 cactcttcac gaccccaggc caccttctgg ataagttcat caaggagttt ctccagccca 1141 acaaatgctt cctagagcag attgacagtg ctgttaacat catccgtaca ttccttaaag 1201 aaaactgctt ccgacaatca acagccaaga tccagattgt ccggggagga tcaaccgcca 1261 aaggcacagc tctgaagact ggctctgatg ccgatctcgt cgtgttccat aactcactta 1321 aaagctacac ctcccaaaaa aacgagcggc acaaaatcgt caaggaaatc catgaacagc 1381 tgaaagcctt ttggagggag aaggaggagg agcttgaagt cagctttgag cctcccaagt 1441 ggaaggctcc cagggtgctg agcttctctc tgaaatccaa agtcctcaac gaaagtgtca 1501 gctttgatgt gcttcctgcc tttaatgcac tgggtcagct gagttctggc tccacaccca 1561 gccccgaggt ttatgcaggg ctcattgatc tgtataaatc ctcggacctc ccgggaggag 1621 agttttctac ctgtttcaca gtcctgcagc gaaacttcat tcgctcccgg cccaccaaac 1681 taaaggattt aattcgcctg gtgaagcact ggtacaaaga gtgtgaaagg aaactgaagc 1741 caaaggggtc tttgccccca aagtatgcct tGGAGCTGCT CACCATCTAT GCCTGGGAGC 1801 AGGGGAGTGG AGTGCCGGAT TTTGACACTG CAGAAGGTTT CCGGACAGTC CTGGAGCTGG 1861 TCACACAATA TCAGCAGCTC TGCATCTTCT GGAAGGTCAA TTACAACTTT GAAGATGAGA 1921 CCGTGAGGAA GTTTCTACTG AGCCAGTTGC AGAAAACCAG GCCTGTGATC TTGGACCCAG 1981 CCGAACCCAC AGGTGACGTG GGTGGAGGGG ACCGTTGGTG TTGGCATCTT CTGGCAAAAG 2041 AAGCAAAGGA ATGGTTATCC TCTCCCTGCT TCAAGGATGG GACTGGAAAC CCAATACCAC 2101 CTTGGAAAGT GCCGGTAAAA GTCATCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 2161 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2221 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACTGACCAA 2281 GCAAATGACC AGGCGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2341 aagatt