Construct: ORF TRCN0000467861

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007166.1_s317c1
Derived from:
ccsbBroadEn_03747
DNA Barcode:
CGTCGCGATAAGTGTACCACCCAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KCMF1 (56888)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467861
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 56888 KCMF1 potassium channel modulator... NM_020122.5 100% 100%
2 human 56888 KCMF1 potassium channel modulator... XM_006712052.3 97.6% 96.6% (many diffs)
3 human 56888 KCMF1 potassium channel modulator... XM_011532990.1 86.6% 86.6% 0_1ins153
4 human 56888 KCMF1 potassium channel modulator... XM_017004511.1 59.8% 59.8% 0_1ins459
5 mouse 74287 Kcmf1 potassium channel modulator... NM_019715.2 94% 97.3% (many diffs)
6 mouse 74287 Kcmf1 potassium channel modulator... NM_001347231.1 81% 83.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1209
ORF length:
1143
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc ccgacatgaa ggtgtcagct gtgatgcatg tttaaaagga aattttcgag
121gtcgcagata taagtgttta atttgctacg attacgatct ttgtgcatct tgttatgaaa
181gtggtgcaac aacaacaagg catacaactg accacccaat gcagtgcata ttaacaaggg
241tagattttga tttatactat ggtggggaag ctttctctgt agagcagcca cagtctttta
301cttgtcccta ttgtggaaaa atgggctata cggagacatc tcttcaagaa catgttactt
361ctgaacatgc agaaacatca acagaagtga tttgtccaat atgtgcagcg ttacctggag
421gcgatcctaa tcatgtcacg gatgactttg cagctcatct tacacttgaa cacagagccc
481ctagagattt agatgaatcg agtggtgttc gacatgtacg tagaatgttt caccctggcc
541ggggattagg aggtcctcgt gctcgtagat caaacatgca ctttactagc agttctactg
601gtggactttc ttcttctcag agttcatatt ctccaagcaa tagggaagcc atggatccta
661tagctgagct tttatctcag ttatcaggag tgagacgttc tgcaggagga cagcttaatt
721cctctggccc ttccgcttct cagttacaac aactgcagat gcagctgcag ctagaacggc
781agcatgccca ggcagcacgg caacaactgg agaccgcacg caacgcaacc cggcgtacta
841acacaagcag tgtcaccact acaatcacac aatccacagc aacaaccaac atagctaata
901cagaaagcag tcagcagact ctacagaatt cccagtttct tttaacaagg ttgaatgatc
961ctaaaatgtc tgaaacggag cgccagtcca tggaaagcga gcgtgcagac cgcagcctgt
1021ttgtccaaga gctccttctg tccactttag tgcgtgaaga gagctcatcc tcagatgagg
1081atgatcgggg ggagatggca gattttggtg ctatgggctg tgtagatatt atgcctttag
1141atgttgcttt agaaaaccta aatTTAAAAG AGAGTAATAA AGGAAATGAG CCTCCACCAC
1201CTCCTCTTTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1261CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1321TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGTCGC GATAAGTGTA CCACCCAGAC
1381GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)