Transcript: Mouse NM_001347231.1

Mus musculus potassium channel modulatory factor 1 (Kcmf1), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Kcmf1 (74287)
Length:
3198
CDS:
451..1443

Additional Resources:

NCBI RefSeq record:
NM_001347231.1
NBCI Gene record:
Kcmf1 (74287)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001347231.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000440536 ACCCGATGCAGTGCATATTAA pLKO_005 446 5UTR 100% 15.000 21.000 N Kcmf1 n/a
2 TRCN0000044732 GTCGCAGATATAAGTGTTTAA pLKO.1 353 5UTR 100% 13.200 18.480 N KCMF1 n/a
3 TRCN0000288978 GTCGCAGATATAAGTGTTTAA pLKO_005 353 5UTR 100% 13.200 18.480 N KCMF1 n/a
4 TRCN0000068923 CCCAGCGTTATCCAATGGTTA pLKO.1 1933 3UTR 100% 4.950 6.930 N Kcmf1 n/a
5 TRCN0000068924 CGGAGATCAAACATGCACTTT pLKO.1 796 CDS 100% 4.950 3.960 N Kcmf1 n/a
6 TRCN0000452932 TCCTGTTAACAAGGTTGAATG pLKO_005 1169 CDS 100% 10.800 7.560 N Kcmf1 n/a
7 TRCN0000068927 GCAGCTCATCTGACACTTGAA pLKO.1 682 CDS 100% 4.950 3.465 N Kcmf1 n/a
8 TRCN0000068925 GCTGTGTAGATATTATGCCTT pLKO.1 1349 CDS 100% 2.640 1.848 N Kcmf1 n/a
9 TRCN0000068926 CTGAGCTGTTATCTCAGTTAT pLKO.1 896 CDS 100% 1.320 0.924 N Kcmf1 n/a
10 TRCN0000044728 GCATATTAACAAGGGTAGATT pLKO.1 458 CDS 100% 5.625 7.875 N KCMF1 n/a
11 TRCN0000288977 GCATATTAACAAGGGTAGATT pLKO_005 458 CDS 100% 5.625 7.875 N KCMF1 n/a
12 TRCN0000044730 GCAGTTCTACTGGTGGACTTT pLKO.1 821 CDS 100% 4.950 3.465 N KCMF1 n/a
13 TRCN0000288980 GCAGTTCTACTGGTGGACTTT pLKO_005 821 CDS 100% 4.950 3.465 N KCMF1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001347231.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03747 pDONR223 100% 81% 83.9% None (many diffs) n/a
2 ccsbBroad304_03747 pLX_304 0% 81% 83.9% V5 (many diffs) n/a
3 TRCN0000467861 CGTCGCGATAAGTGTACCACCCAG pLX_317 4.8% 81% 83.9% V5 (many diffs) n/a
Download CSV