Construct: ORF TRCN0000467882
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001977.1_s317c1
- Derived from:
- ccsbBroadEn_09108
- DNA Barcode:
- CCAGCGGACGCTTTCATTTAGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SH3BGRL2 (83699)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467882
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83699 | SH3BGRL2 | SH3 domain binding glutamat... | NM_031469.4 | 99.6% | 99% | 116T>C |
| 2 | human | 83699 | SH3BGRL2 | SH3 domain binding glutamat... | XM_011536165.2 | 88% | 85% | (many diffs) |
| 3 | mouse | 212531 | Sh3bgrl2 | SH3 domain binding glutamic... | NM_172507.5 | 88.1% | 92.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 390
- ORF length:
- 321
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtcatccgc GTGTTCATCG CCTCTTCCTC GGGCTTCGTG GCGATAAAGA 121 AGAAGCAGCA AGATGTGGTT AGATTTCTGG AAGCCAACAA GATAGAGTTT GAGGAGGTGG 181 ATACCACAAT GTCAGAAGAA CAGAGGCAAT GGATGTACAA AAACGTCCCC CCGGAAAAGA 241 AACCCACTCA GGGCAACCCC CTGCCACCTC AGATATTTAA TGGCGACCGA TACTGTGGAG 301 ATTATGACAG TTTTTTTGAA TCCAAGGAAA GCAACACAGT CTTTTCATTT TTAGGCCTGA 361 AACCACGGTT GGCATCAAAG GCAGAACCTT TGCCAACTTT CTTGTACAAA GTGGTTGATA 421 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 481 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCAGC 541 GGACGCTTTC ATTTAGCGCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 601 tgaaagatt