Construct: ORF TRCN0000467888

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015066.1_s317c1
Derived from:
ccsbBroadEn_09705
DNA Barcode:
ATGGTCTCAACACTATACTAAACG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CNKSR3 (154043)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467888
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 154043 CNKSR3 CNKSR family member 3 NM_173515.4 99.9% 100% 168T>C
2 human 154043 CNKSR3 CNKSR family member 3 XM_011535485.3 97.7% 97.2% (many diffs)
3 human 154043 CNKSR3 CNKSR family member 3 NM_001368116.1 96.9% 96.2% (many diffs)
4 human 154043 CNKSR3 CNKSR family member 3 NM_001368117.1 94.1% 80.8% 168T>C;1367_1368ins88;1569_1570insTGACTCAC
5 human 154043 CNKSR3 CNKSR family member 3 NM_001368118.1 85.5% 85.5% 0_1ins240
6 human 154043 CNKSR3 CNKSR family member 3 NM_001368119.1 68.4% 68.4% 0_1ins525
7 mouse 215748 Cnksr3 Cnksr family member 3 NM_172546.2 85.7% 92.2% (many diffs)
8 mouse 215748 Cnksr3 Cnksr family member 3 XM_011243079.1 80.4% 86.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1731
ORF length:
1665
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga acccgtgacc aagtggagcc ccaaacaagt ggtggactgg actagagggt
121tggatgactg cctgcaacaa tatgtccaca agtttgaacg agagaagatc aacggcgagc
181agctgctgca gatttcccat caggacctgg aggagctggg ggtcacacgg atcggacacc
241aggagcttgt gttggaggct gtggaccttc tctgtgcact gaattatggc ctcgaaactg
301ataacatgaa gaacttggtt ctgaaactga gagcatcttc ccacaattta cagaattaca
361taagtagccg acggaaaagt cccgcttacg atggcaacac ctcccgcaag gcccccaatg
421agttcctgac ctcggtggtg gagctcatcg gcgccgccaa ggccctgctg gcgtggctgg
481accgggctcc gtttacaggg atcactgatt tctcagtcac gaagaacaaa attatccagc
541tttgcttgga cctgaccact acagtccaga aggattgctt tgtagcggaa atggaggata
601aagttttaac tgtggtcaag gttttaaatg gcatctgtga caaaacaatc cgatctacca
661cagatcctgt gatgagccag tgtgcatgtc tggaggaagt tcacttacca aacattaaac
721ctggggaagg cctgggcatg tacatcaaat caacctatga tgggttacac gtgattactg
781gaaccacaga aaattctcct gcagacagat ctcagaagat tcatgctggt gacgaagtca
841ttcaagttaa tcagcaaact gtggtgggat ggcagctgaa aaatctggtg aagaaattga
901gagagaatcc caccggagtt gtgttactgc ttaagaagcg ccccaccggg tctttcaact
961ttactcctgc tcccctgaaa aacctacggt ggaagccacc tcttgtacag acctcacctc
1021cacccgcgac aacccagtcc cctgaaagca ctatggatac ctcactgaag aaggagaagt
1081cagccatcct ggatctttat attcctcctc cgcctgctgt tccctactct ccccgggatg
1141agaatggcag ttttgtttat ggagggtcca gtaagtgcaa acaaccattg cctggtccta
1201agggttcaga gtccccgaat tccttcttgg accaggaaag ccggagacga agattcacca
1261ttgcagactc ggatcagttg cctgggtact cggtggaaac caacattctg cccacaaaaa
1321tgagagagaa aacaccaTCT TATGGCAAGC CACGGCCTTT GTCCATGCCT GCTGATGGGA
1381ACTGGATGGG GATTGTGGAC CCTTTTGCCA GACCTCGAGG TCATGGCAGG AAAGGGGAGG
1441ATGCCCTTTG CCGGTATTTC AGTAACGAGC GGATTCCTCC GATCATTGAA GAGAGCTCCT
1501CTCCCCCATA CCGGTTCTCC AGACCCACGA CCGAGCGGCA TCTGGTCCGG GGTGCGGACT
1561ACATCCGAGG AAGCAGGTGC TACATCAACT CAGATCTCCA CAGCAGCGCC ACGATTCCAT
1621TCCAGGAGGA AGGGACCAAA AAGAAATCTG GCTCCTCAGC TACGAAGTCC TCGTCCACAG
1681AACCGTCCCT CCTGGTCAGC TGGTTTACGC GCCTCAAACT GTTGACTCAC TGCCCAACTT
1741TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1801CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1861CTTGTGGAAA GGACGAATGG TCTCAACACT ATACTAAACG ACGCGTTAAG TCgacaatca
1921acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)