Construct: ORF TRCN0000467888
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015066.1_s317c1
- Derived from:
- ccsbBroadEn_09705
- DNA Barcode:
- ATGGTCTCAACACTATACTAAACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNKSR3 (154043)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467888
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 154043 | CNKSR3 | CNKSR family member 3 | NM_173515.4 | 99.9% | 100% | 168T>C |
| 2 | human | 154043 | CNKSR3 | CNKSR family member 3 | XM_011535485.3 | 97.7% | 97.2% | (many diffs) |
| 3 | human | 154043 | CNKSR3 | CNKSR family member 3 | NM_001368116.1 | 96.9% | 96.2% | (many diffs) |
| 4 | human | 154043 | CNKSR3 | CNKSR family member 3 | NM_001368117.1 | 94.1% | 80.8% | 168T>C;1367_1368ins88;1569_1570insTGACTCAC |
| 5 | human | 154043 | CNKSR3 | CNKSR family member 3 | NM_001368118.1 | 85.5% | 85.5% | 0_1ins240 |
| 6 | human | 154043 | CNKSR3 | CNKSR family member 3 | NM_001368119.1 | 68.4% | 68.4% | 0_1ins525 |
| 7 | mouse | 215748 | Cnksr3 | Cnksr family member 3 | NM_172546.2 | 85.7% | 92.2% | (many diffs) |
| 8 | mouse | 215748 | Cnksr3 | Cnksr family member 3 | XM_011243079.1 | 80.4% | 86.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1731
- ORF length:
- 1665
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga acccgtgacc aagtggagcc ccaaacaagt ggtggactgg actagagggt 121 tggatgactg cctgcaacaa tatgtccaca agtttgaacg agagaagatc aacggcgagc 181 agctgctgca gatttcccat caggacctgg aggagctggg ggtcacacgg atcggacacc 241 aggagcttgt gttggaggct gtggaccttc tctgtgcact gaattatggc ctcgaaactg 301 ataacatgaa gaacttggtt ctgaaactga gagcatcttc ccacaattta cagaattaca 361 taagtagccg acggaaaagt cccgcttacg atggcaacac ctcccgcaag gcccccaatg 421 agttcctgac ctcggtggtg gagctcatcg gcgccgccaa ggccctgctg gcgtggctgg 481 accgggctcc gtttacaggg atcactgatt tctcagtcac gaagaacaaa attatccagc 541 tttgcttgga cctgaccact acagtccaga aggattgctt tgtagcggaa atggaggata 601 aagttttaac tgtggtcaag gttttaaatg gcatctgtga caaaacaatc cgatctacca 661 cagatcctgt gatgagccag tgtgcatgtc tggaggaagt tcacttacca aacattaaac 721 ctggggaagg cctgggcatg tacatcaaat caacctatga tgggttacac gtgattactg 781 gaaccacaga aaattctcct gcagacagat ctcagaagat tcatgctggt gacgaagtca 841 ttcaagttaa tcagcaaact gtggtgggat ggcagctgaa aaatctggtg aagaaattga 901 gagagaatcc caccggagtt gtgttactgc ttaagaagcg ccccaccggg tctttcaact 961 ttactcctgc tcccctgaaa aacctacggt ggaagccacc tcttgtacag acctcacctc 1021 cacccgcgac aacccagtcc cctgaaagca ctatggatac ctcactgaag aaggagaagt 1081 cagccatcct ggatctttat attcctcctc cgcctgctgt tccctactct ccccgggatg 1141 agaatggcag ttttgtttat ggagggtcca gtaagtgcaa acaaccattg cctggtccta 1201 agggttcaga gtccccgaat tccttcttgg accaggaaag ccggagacga agattcacca 1261 ttgcagactc ggatcagttg cctgggtact cggtggaaac caacattctg cccacaaaaa 1321 tgagagagaa aacaccaTCT TATGGCAAGC CACGGCCTTT GTCCATGCCT GCTGATGGGA 1381 ACTGGATGGG GATTGTGGAC CCTTTTGCCA GACCTCGAGG TCATGGCAGG AAAGGGGAGG 1441 ATGCCCTTTG CCGGTATTTC AGTAACGAGC GGATTCCTCC GATCATTGAA GAGAGCTCCT 1501 CTCCCCCATA CCGGTTCTCC AGACCCACGA CCGAGCGGCA TCTGGTCCGG GGTGCGGACT 1561 ACATCCGAGG AAGCAGGTGC TACATCAACT CAGATCTCCA CAGCAGCGCC ACGATTCCAT 1621 TCCAGGAGGA AGGGACCAAA AAGAAATCTG GCTCCTCAGC TACGAAGTCC TCGTCCACAG 1681 AACCGTCCCT CCTGGTCAGC TGGTTTACGC GCCTCAAACT GTTGACTCAC TGCCCAACTT 1741 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1801 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1861 CTTGTGGAAA GGACGAATGG TCTCAACACT ATACTAAACG ACGCGTTAAG TCgacaatca 1921 acctctggat tacaaaattt gtgaaagatt