Construct: ORF TRCN0000467896
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005183.1_s317c1
- Derived from:
- ccsbBroadEn_01825
- DNA Barcode:
- TGCAGCAAGATTTACTCAAGTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC30A2 (7780)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467896
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7780 | SLC30A2 | solute carrier family 30 me... | NM_032513.5 | 100% | 100% | |
| 2 | human | 7780 | SLC30A2 | solute carrier family 30 me... | NM_001004434.3 | 86.8% | 86.8% | 270_416del |
| 3 | mouse | 230810 | Slc30a2 | solute carrier family 30 (z... | NM_001346578.1 | 68.6% | 54.9% | (many diffs) |
| 4 | mouse | 230810 | Slc30a2 | solute carrier family 30 (z... | XM_006538755.3 | 68.6% | 54.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1035
- ORF length:
- 969
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggccaaggag aagcagcatc tgttggacgc caggccggca atccggtcat 121 acacgggatc tctgtggcag gaaggggctg gctggattcc tctgccccga cctggcctgg 181 acttgcaggc cattgagctg gctgcccaga gcaaccatca ctgccatgct cagaagggtc 241 ctgacagtca ctgtgacccc aagaagggga aggcccagcg ccagctgtat gtagcctctg 301 ccatctgcct gttgttcatg atcggagaag tcgttgagat cttgggagcc ctggtctctg 361 tactgtccat ctgggtcgtg acgggggtac tggtgtacct ggctgtggag cggctgatct 421 ctggggacta tgaaattgac ggggggacca tgctgatcac gtcgggctgc gctgtggctg 481 tgaacatcat aatggggttg acccttcacc agtctggcca tgggcacagc cacggcacca 541 ccaaccagca ggaggagaac cccagcgtcc gagctgcctt catccatgtg atcggcgact 601 ttatgcagag catgggtgtc ctagtggcag cctatatttt atacttcaag ccagaataca 661 agtatgtaga ccccatctgc accttcgtct tctccatcct ggtcctgggg acaaccttga 721 ccatcctgag agatgtgatc ctggtgttga tggaagggac ccccaagggc gttgacttca 781 cagctgttcg tgatctgctg ctgtcggtgg agggggtaga agcccTGCAC AGCCTGCATA 841 TCTGGGCACT GACGGTGGCC CAGCCTGTTC TGTCTGTCCA CATCGCCATT GCTCAGAATA 901 CAGACGCCCA GGCTGTGCTG AAGACAGCCA GCAGCCGCCT CCAAGGGAAG TTCCACTTCC 961 ACACCGTGAC CATCCAGATC GAGGACTACT CGGAGGACAT GAAGGACTGT CAGGCATGCC 1021 AGGGCCCCTC AGACTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1081 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1141 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TGCAGCAAGA TTTACTCAAG 1201 TCCCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt