Construct: ORF TRCN0000467898
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013064.1_s317c1
- Derived from:
- ccsbBroadEn_06972
- DNA Barcode:
- CTTAAGTATCTAGTATCCATGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A1 (6576)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467898
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6576 | SLC25A1 | solute carrier family 25 me... | NM_005984.5 | 99.8% | 99.6% | 13C>G |
| 2 | human | 6576 | SLC25A1 | solute carrier family 25 me... | NM_001256534.2 | 94% | 90% | (many diffs) |
| 3 | human | 6576 | SLC25A1 | solute carrier family 25 me... | NM_001287387.2 | 66.8% | 66.8% | 0_1ins309 |
| 4 | human | 6576 | SLC25A1 | solute carrier family 25 me... | NR_046298.2 | 46.6% | (many diffs) | |
| 5 | mouse | 13358 | Slc25a1 | solute carrier family 25 (m... | NM_153150.2 | 88.1% | 93.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 999
- ORF length:
- 933
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cgcgcccggc gccccgcgcg ctctggcggc cgccgcgccc gcgtccggga 121 aggccaagct gacgcacccg gggaaggcga tcctggcagg cggcctggcg ggtggcatcg 181 agatctgcat caccttcccc accgagtacg tgaagacgca gctgcagctg gacgagcgct 241 cgcacccgcc gcggtaccgg ggcatcgggg actgcgtgcg gcagacggtt cgcagccatg 301 gcgtcctggg cctgtaccgc ggccttagct ccctgctcta cggttccatc cccaaggcgg 361 ccgtcaggtt tggaatgttc gagttcctca gcaaccacat gcgggatgcc cagggacggc 421 tggacagcac gcgtgggctg ctgtgcggcc tgggcgctgg cgtggccgag gccgtggtgg 481 tcgtgtgccc catggagacc atcaaggtga agttcatcca cgaccagacc tccccaaacc 541 ccaagtacag aggattcttc cacggggtta gggagattgt gcgggaacaa gggctgaagg 601 ggacgtacca gggcctcaca gccactgtcc tgaagcaggg ctcgaaccag gccatccgct 661 tcttcgtcat gacctccctg cgcaactggt accgagggga caaccccaac aagcccatga 721 accctctgat cactggggtc ttcggagcta ttgcaggcgc agccagtgtc tttGGAAACA 781 CTCCTCTGGA TGTGATTAAG ACCCGGATGC AGGGCCTGGA GGCGCACAAA TACCGGAACA 841 CGTGGGACTG CGGCTTGCAG ATCCTGAAGA AGGAGGGGCT CAAGGCATTC TACAAGGGCA 901 CTGTCCCCCG CCTGGGCCGG GTCTGCCTGG ATGTGGCCAT AGTGTTTGTC ATCTATGATG 961 AAGTGGTGAA GCTGCTCAAC AAAGTGTGGA AGACGGACTA CCCAACTTTC TTGTACAAAG 1021 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1081 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1141 ACGACTTAAG TATCTAGTAT CCATGTAAAC GCGTTAAGTC gacaatcaac ctctggatta 1201 caaaatttgt gaaagatt