Construct: ORF TRCN0000467922
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016827.1_s317c1
- Derived from:
- ccsbBroadEn_03414
- DNA Barcode:
- CTCCGACATGTTCATGGGGTGAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- YIPF1 (54432)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467922
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54432 | YIPF1 | Yip1 domain family member 1 | NM_018982.5 | 100% | 100% | |
| 2 | human | 54432 | YIPF1 | Yip1 domain family member 1 | NR_135075.2 | 59.3% | (many diffs) | |
| 3 | human | 54432 | YIPF1 | Yip1 domain family member 1 | NR_036640.2 | 55.5% | 1_134del;1053_1652del | |
| 4 | human | 54432 | YIPF1 | Yip1 domain family member 1 | NR_036639.2 | 49% | 1_354del;1273_1872del | |
| 5 | mouse | 230584 | Yipf1 | Yip1 domain family, member 1 | NM_145550.3 | 83.3% | 87.5% | (many diffs) |
| 6 | mouse | 230584 | Yipf1 | Yip1 domain family, member 1 | XM_006502968.3 | 76.3% | 79.7% | (many diffs) |
| 7 | mouse | 230584 | Yipf1 | Yip1 domain family, member 1 | NM_001205156.1 | 75.6% | 79% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 984
- ORF length:
- 918
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agcagtagat gacttgcaat ttgaagaatt tggcaatgca gccacttctc 121 tgacagcaaa cccagatgcc accacagtaa acattgagga tcctggtgaa accccaaaac 181 atcagccagg atccccaaga ggctcaggaa gagaagaaga tgatgagtta ctgggaaatg 241 atgactctga caaaactgag ttacttgctg gacagaagaa aagctccccc ttctggacat 301 ttgaatacta ccaaacattc tttgatgtgg acacctacca ggtctttgac agaattaaag 361 gatctctttt gccaataccc gggaaaaact ttgtgaggtt atatatccgc agcaatccag 421 atctctatgg ccccttttgg atatgtgcca cgttggtctt tgccatagca attagtggga 481 atctttccaa cttcttgatc catctgggag agaagacgta ccattatgtg cccgaattcc 541 gaaaagtgtc catagcagct accaTCATCT ATGCCTATGC CTGGCTGGTT CCTCTTGCAC 601 TCTGGGGTTT CCTCATGTGG AGAAACAGCA AAGTTATGAA CATCGTCTCC TATTCATTTC 661 TGGAGATTGT GTGTGTCTAT GGATATTCCC TCTTCATTTA TATCCCCACC GCAATACTGT 721 GGATTATCCC CCAGAAAGCT GTTCGTTGGA TTCTAGTCAT GATTGCCCTG GGCATCTCAG 781 GATCTCTCTT GGCAATGACA TTTTGGCCAG CTGTTCGTGA GGATAACCGA CGCGTTGCAT 841 TGGCCACAAT TGTGACAATT GTGTTGCTCC ATATGCTGCT TTCTGTGGGC TGCTTGGCAT 901 ACTTTTTTGA TGCACCAGAG ATGGACCATC TCCCAACAAC TACAGCTACT CCAAACCAAA 961 CAGTTGCTGC AGCCAAGTCC AGCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1021 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1081 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC TCCGACATGT 1141 TCATGGGGTG AAGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1201 att