Construct: ORF TRCN0000467936
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018514.1_s317c1
- Derived from:
- ccsbBroadEn_08492
- DNA Barcode:
- CATGCTGGCGGCGACGTATTATGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHDC8A (55220)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467936
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55220 | KLHDC8A | kelch domain containing 8A | NM_001271863.1 | 99.7% | 99.7% | 204C>A;479C>A;717C>A |
| 2 | human | 55220 | KLHDC8A | kelch domain containing 8A | NM_001271864.1 | 99.7% | 99.7% | 204C>A;479C>A;717C>A |
| 3 | human | 55220 | KLHDC8A | kelch domain containing 8A | NM_001271865.1 | 99.7% | 99.7% | 204C>A;479C>A;717C>A |
| 4 | human | 55220 | KLHDC8A | kelch domain containing 8A | NM_018203.3 | 99.7% | 99.7% | 204C>A;479C>A;717C>A |
| 5 | human | 55220 | KLHDC8A | kelch domain containing 8A | XM_024448121.1 | 99.7% | 99.7% | 204C>A;479C>A;717C>A |
| 6 | mouse | 213417 | Klhdc8a | kelch domain containing 8A | NM_144810.5 | 88.8% | 95.4% | (many diffs) |
| 7 | mouse | 213417 | Klhdc8a | kelch domain containing 8A | XM_006529354.3 | 88.8% | 95.4% | (many diffs) |
| 8 | mouse | 213417 | Klhdc8a | kelch domain containing 8A | XM_006529355.3 | 70.5% | 75.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1116
- ORF length:
- 1050
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggtgcctaac gtcaaggact tccagtggaa gcgcctggcg ccactgccca 121 gccgccgggt ctactgctcc ctgctggaga ccgggggcca ggtctatgcc atcgggggat 181 gtgacgacaa cggcgtcccc atggactgct tcgaggtcta ctccccggag gccgaccagt 241 ggaccgcctt gccccggctg cccacagcac gggcgggggt ggccgtcacc gccctgggga 301 agcggatcat ggtgattggg ggcgtgggca ccaatcagct gcccctgaag gtcgtggaga 361 tgtacaacat cgatgagggc aagtggaaga agaggagcat gctgcgtgag gccgccatgg 421 gcatttctgt cacggccaaa gattaccgag tatatgcggc aggcgggatg ggcctggacc 481 tacgtccaca caaccacctc caacactatg acatgctgaa ggacatgtgg gtgtccctag 541 cacacatgcc caccccgaga tatgctgcca cctccttcct ccgaggctcc aagatctacg 601 tgctgggggg acgacagtcc aagtacgcgg tcaacgcttt cgaggtcttt gacatcgaga 661 ctcgctcctg gaccaagttt cccaacattc cctataagcg ggccttctcc agctttgtga 721 ccctggacaa ccacttgtac agcctaggag gccTGCGGCA AGGTCGCCTC TACCGGCAGC 781 CAAAGTTCCT GCGGACGATG GACGTGTTCG ACATGGAACA GGGGGGATGG CTGAAGATGG 841 AACGATCGTT CTTCCTCAAG AAGCGGCGGG CAGATTTTGT GGCTGGCTCT CTGAGTGGAC 901 GGGTCATAGT GGCTGGGGGA CTTGGGAATC AACCCACTGT CCTGGAGACG GCGGAAGCAT 961 TCCACCCAGG GAAGAACAAA TGGGAGATCC TCCCTGCCAT GCCCACACCC CGCTGTGCCT 1021 GCTCCAGCAT AGTCGTCAAG AACTGCCTCC TCGCCGTGGG AGGTGTCAAC CAGGGTCTGA 1081 GTGACGCAGT GGAGGCCCTG TGTGTCTCTG ACTCCTACCC AACTTTCTTG TACAAAGTGG 1141 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1201 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1261 ACATGCTGGC GGCGACGTAT TATGGACGCG TTAAGTCgac aatcaacctc tggattacaa 1321 aatttgtgaa agatt