Construct: ORF TRCN0000467938
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005538.1_s317c1
- Derived from:
- ccsbBroadEn_10486
- DNA Barcode:
- TGTGCTAAACGTAAGGACCGCGAC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZBTB8A (653121)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467938
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 653121 | ZBTB8A | zinc finger and BTB domain ... | NM_001040441.3 | 99.8% | 99.5% | 8T>A;1321A>T |
| 2 | human | 653121 | ZBTB8A | zinc finger and BTB domain ... | NM_001291496.2 | 77% | 75.2% | 8T>A;993_994ins181;1020_1021ins122 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1389
- ORF length:
- 1323
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaactcctct catcagtctc acctcctgca gcaactgaac gagcagcgca 121 ggcaagatgt attttgtgac tgcagtattc tagttgaagg gaaggtcttc aaagcacatc 181 gaaatgtatt attcgctagt agcggctact ttaaaatgct tctttctcag aattcaaagg 241 agacgagtca gccaaccaca gctacatttc aggctttctc ccctgacact tttacagtta 301 tcttggactt cgtatattct ggcaaactgt ctcttactgg tcagaatgtc atagaagtga 361 tgtcggctgc tagcttcctt cagatgactg atgtcataag tgtatgtaag acttttatta 421 aatcttcctt agacattagt gagaaagaaa aagatcgcta tttcagtctc tcagataaag 481 atgccaattc taatggtgta gaacgttcct ctttttatag tggtggctgg caagaaggaa 541 gcagttctcc acgttctcac ctaagcccag agcaaggaac aggtataata agtggaaaat 601 cttggaataa gtataattat catccagcct cccagaagaa tactcaacaa cccttggcca 661 agcatgaacc aaggaaagag tccattaaaa agaccaaaca tttgagattg tcacagcctt 721 ctgaagttac tcattataag tcaagcaaac gagaagtacg aacatctgat tcttccagcc 781 atgtttccca gtctgaagaa caagcacaga ttgatgctga aatggactct actcctgttg 841 gctatcagta cggtcaagga tctgatgtca catccaaaag ctttccagat gatctgcctc 901 ggatgcgatt caagtgcccg tactgcacac atgtggtgaa gcggaaggca gacctaaagc 961 gccaccTTCG TTGTCATACA GGAGAAAGGC CCTATCCATG TCAAGCTTGT GGAAAAAGAT 1021 TTAGCAGGCT AGACCATCTA AGTAGCCATT TTCGAACAAT TCACCAGGCA TGTAAACTCA 1081 TCTGCAGAAA ATGTAAACGT CATGTGACAG ATCTAACAGG GCAAGTGGTA CAGGAGGGAA 1141 CCAGGCGCTA CAGACTGTGT AATGAGTGTC TTGCAGAATT TGGCATAGAC AGCCTCCCCA 1201 TTGACTTGGA AGCTGAACAA CATCTTATGT CCCCATCAGA TGGAGATAAG GATTCCAGAT 1261 GGCACTTGAG TGAAGATGAG AATAGATCCT ATGTGGAGAT TGTAGAAGAT GGGTCTGCTG 1321 ATCTGGTCAT CCAACAGGTT GATGATAGTG AAGAAGAAGA AGAAAAAGAA ATTAAGCCCA 1381 ACATTTGGTA CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC 1441 TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT 1501 TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGTGCTA AACGTAAGGA CCGCGACACG 1561 CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt