Construct: ORF TRCN0000467953
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014304.1_s317c1
- Derived from:
- ccsbBroadEn_01170
- DNA Barcode:
- GCAACCCAGAAGGATTGTCTCAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDZK1 (5174)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467953
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5174 | PDZK1 | PDZ domain containing 1 | NM_001201325.2 | 100% | 100% | |
| 2 | human | 5174 | PDZK1 | PDZ domain containing 1 | NM_002614.4 | 100% | 100% | |
| 3 | human | 5174 | PDZK1 | PDZ domain containing 1 | NM_001371359.1 | 97.1% | 96.9% | (many diffs) |
| 4 | human | 5174 | PDZK1 | PDZ domain containing 1 | XM_024447616.1 | 96.2% | 96.2% | 1_60del |
| 5 | human | 5174 | PDZK1 | PDZ domain containing 1 | XM_011509616.3 | 93.8% | 93.3% | (many diffs) |
| 6 | human | 5174 | PDZK1 | PDZ domain containing 1 | XM_024447619.1 | 93.5% | 93.3% | (many diffs) |
| 7 | human | 5174 | PDZK1 | PDZ domain containing 1 | XM_024447620.1 | 93.5% | 93.3% | (many diffs) |
| 8 | human | 5174 | PDZK1 | PDZ domain containing 1 | NM_001201326.1 | 78.6% | 78.6% | 457_458ins333 |
| 9 | human | 100034743 | PDZK1P1 | PDZ domain containing 1 pse... | NR_111936.1 | 64.2% | (many diffs) | |
| 10 | human | 5174 | PDZK1 | PDZ domain containing 1 | NM_001371361.1 | 60.1% | 60.1% | 0_1ins621 |
| 11 | human | 5174 | PDZK1 | PDZ domain containing 1 | XM_017001467.2 | 60.1% | 60.1% | 0_1ins621 |
| 12 | human | 5174 | PDZK1 | PDZ domain containing 1 | XR_002956801.1 | 44.7% | (many diffs) | |
| 13 | human | 5174 | PDZK1 | PDZ domain containing 1 | XR_002956798.1 | 43.9% | 1_1626del;3184_3545del | |
| 14 | human | 5174 | PDZK1 | PDZ domain containing 1 | XR_002956800.1 | 42.4% | 1_1633del;2623_2735del;3304_3665del | |
| 15 | human | 5174 | PDZK1 | PDZ domain containing 1 | XR_002956799.1 | 40.3% | 1_1633del;2623_2735del;3304_3859del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1623
- ORF length:
- 1557
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ctccaccttc aacccccgag aatgtaaact gtccaagcaa gaagggcaaa 121 actatggctt cttcctgcga attgagaagg acaccgaggg ccacctggtc cgggtggttg 181 agaagtgtag cccagcagag aaggctggcc ttcaagatgg agacagagtt cttaggatca 241 atggtgtctt tgtggacaaa gaagaacata tgcaggttgt ggatctggtc agaaagagtg 301 ggaattcagt gactttacta gttctggatg gggattccta tgagaaagca gtgaaaacac 361 gggtggactt gaaagagttg ggtcaaagtc agaaggagca aggtttgagt gataatatac 421 tttcccctgt gatgaatgga ggtgtgcaaa cttggaccca gccccggctc tgctatctcg 481 tgaaggaagg aggcagctat ggcttctctc tgaaaactgt ccaaggtaaa aagggggtgt 541 acatgactga tattacacct caaggtgtgg ctatgagagc tggagttctg gctgatgatc 601 acttgattga agtgaatgga gagaatgtag aggatgccag ccatgaggaa gtggttgaaa 661 aggtgaagaa gtcaggaagc cgtgtcatgt tcctgctggt ggacaaagaa actgacaagc 721 gtcatgttga gcagaagata caattcaaaa gagaaacagc cagtttgaaa ctgttacccc 781 accagccccg aattgtggag atgaagaaag gaagcaatgg ctatggtttc tatctgaggg 841 caggctcaga acagaaaggt caaatcatca aggacataga ttctggaagt ccagcagagg 901 aggctggctt gaagaacaat gatctggtag ttgctgtcaa cggcgagtct gtggaaaccc 961 tggatcatga cagtgtggta gaaatgatta gaaagggtgg agatcagact tcactgttgg 1021 tggtagacaa agagacggac aacatgtaca gactggctca tttttctcca tttctctact 1081 atcaaagtca agaactgccc aatggctctg tcaaggaggc tccagctcct actcccactt 1141 ctctggaagt ctcaagtcca ccagatacta cagaggaagt agatcataag cctaaactct 1201 gcaggctggc taaaggtgaa aatggctatg gctttcactt aaatgcgatt cggggTCTGC 1261 CAGGCTCATT CATCAAAGAG GTACAGAAGG GCGGTCCTGC TGACTTGGCT GGGCTAGAGG 1321 ATGAGGATGT CATCATTGAA GTGAATGGGG TGAATGTGCT AGATGAACCC TATGAGAAGG 1381 TGGTGGATAG AATCCAGAGC AGTGGGAAGA ATGTCACACT TCTAGTCTGT GGAAAGAAGG 1441 CCTATGATTA TTTCCAAGCT AAGAAAATCC CTATTGTTTC CTCCCTGGCT GATCCACTTG 1501 ACACCCCTCC AGATTCTAAA GAAGGAATAG TGGTGGAGTC AAACCATGAC TCGCACATGG 1561 CAAAAGAACG GGCCCACAGT ACAGCCTCAC ATTCTTCTTC CAATTCTGAA GATACAGAGA 1621 TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1681 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1741 GGCTTTATAT ATCTTGTGGA AAGGACGAGC AACCCAGAAG GATTGTCTCA GGACGCGTTA 1801 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt