Construct: ORF TRCN0000467977
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015891.1_s317c1
- Derived from:
- ccsbBroadEn_06212
- DNA Barcode:
- GACATCATACTCCAATGCCCTACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FGL1 (2267)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467977
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2267 | FGL1 | fibrinogen like 1 | NM_004467.4 | 99.8% | 100% | 42G>T |
| 2 | human | 2267 | FGL1 | fibrinogen like 1 | NM_147203.2 | 99.8% | 100% | 42G>T |
| 3 | human | 2267 | FGL1 | fibrinogen like 1 | NM_201552.1 | 99.8% | 100% | 42G>T |
| 4 | human | 2267 | FGL1 | fibrinogen like 1 | NM_201553.1 | 99.8% | 100% | 42G>T |
| 5 | human | 2267 | FGL1 | fibrinogen like 1 | XM_017013232.1 | 99.8% | 100% | 42G>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1002
- ORF length:
- 936
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aaaggtgttc agtttcatcc ttgttaccac cgctcttaca atgggcaggg 121 aaatttcggc gctcgaggac tgtgcccagg agcagatgcg gctcagagcc caggtgcgcc 181 tgcttgagac ccgggtcaaa cagcaacagg tcaagatcaa gcagcttttg caggagaatg 241 aagtccagtt ccttgataaa ggagatgaga atactgtcat tgatcttgga agcaagaggc 301 agtatgcaga ttgttcagag attttcaatg atgggtataa gctcagtgga ttttacaaaa 361 tcaaacctct ccagagccca gcagaatttt ctgtttattg tgacatgtcc gatggaggag 421 gatggactgt aattcagaga cgatctgatg gcagtgaaaa ctttaacaga ggatggaaag 481 actatgaaaa tggctttgga aattttgtcc aaaaacatgg tgaatattgg ctgggcaata 541 aaaatcttca cttcttgacc actcaagaag actacacttt aaaaatcgac cttgcagatt 601 ttgaaaaaaa tagccgttat gcacaatata agaatttcaa agttGGAGAT GAAAAGAATT 661 TCTACGAGTT GAATATTGGG GAATATTCTG GAACAGCTGG AGATTCCCTT GCGGGGAATT 721 TTCATCCTGA GGTGCAGTGG TGGGCTAGTC ACCAAAGAAT GAAATTCAGC ACGTGGGACA 781 GAGATCATGA CAACTATGAA GGGAACTGCG CAGAAGAAGA TCAGTCTGGC TGGTGGTTTA 841 ACAGGTGTCA CTCTGCAAAC CTGAATGGTG TATACTACAG CGGCCCCTAC ACGGCTAAAA 901 CAGACAATGG GATTGTCTGG TACACCTGGC ATGGGTGGTG GTATTCTCTG AAATCTGTGG 961 TTATGAAAAT TAGGCCAAAT GATTTTATTC CAAATGTAAT TTACCCAACT TTCTTGTACA 1021 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1081 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1141 AGGACGAGAC ATCATACTCC AATGCCCTAC GACGCGTTAA GTCgacaatc aacctctgga 1201 ttacaaaatt tgtgaaagat t