Construct: ORF TRCN0000468000
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010004.1_s317c1
- Derived from:
- ccsbBroadEn_07127
- DNA Barcode:
- AACGAGCTTTAACTCGGGGGACCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VEGFC (7424)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468000
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7424 | VEGFC | vascular endothelial growth... | NM_005429.5 | 99.9% | 99.7% | 1240A>C |
| 2 | mouse | 22341 | Vegfc | vascular endothelial growth... | NM_009506.2 | 86.7% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1323
- ORF length:
- 1257
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca cttgctgggc ttcttctctg tggcgtgttc tctgctcgcc gctgcgctgc 121 tcccgggtcc tcgcgaggcg cccgccgccg ccgccgcctt cgagtccgga ctcgacctct 181 cggacgcgga gcccgacgcg ggcgaggcca cggcttatgc aagcaaagat ctggaggagc 241 agttacggtc tgtgtccagt gtagatgaac tcatgactgt actctaccca gaatattgga 301 aaatgtacaa gtgtcagcta aggaaaggag gctggcaaca taacagagaa caggccaacc 361 tcaactcaag gacagaagag actataaaat ttgctgcagc acattataat acagagatct 421 tgaaaagtat tgataatgag tggagaaaga ctcaatgcat gccacgggag gtgtgtatag 481 atgtggggaa ggagtttgga gtcgcgacaa acaccttctt taaacctcca tgtgtgtccg 541 tctacagatg tgggggttgc tgcaatagtg aggggctgca gtgcatgaac accagcacga 601 gctacctcag caagacgtta tttgaaatta cagtgcctct ctctcaaggc cccaaaccag 661 taacaatcag ttttgccaat cacacttcct gccgatgcat gtctaaactg gatgtttaca 721 gacaagttca ttccattatt agacgttccc tgccagcaac actaccacag tgtcaggcag 781 cgaacaagac ctgccccacc aattacatgt ggaataatca catctgcaga tgcctggcTC 841 AGGAAGATTT TATGTTTTCC TCGGATGCTG GAGATGACTC AACAGATGGA TTCCATGACA 901 TCTGTGGACC AAACAAGGAG CTGGATGAAG AGACCTGTCA GTGTGTCTGC AGAGCGGGGC 961 TTCGGCCTGC CAGCTGTGGA CCCCACAAAG AACTAGACAG AAACTCATGC CAGTGTGTCT 1021 GTAAAAACAA ACTCTTCCCC AGCCAATGTG GGGCCAACCG AGAATTTGAT GAAAACACAT 1081 GCCAGTGTGT ATGTAAAAGA ACCTGCCCCA GAAATCAACC CCTAAATCCT GGAAAATGTG 1141 CCTGTGAATG TACAGAAAGT CCACAGAAAT GCTTGTTAAA AGGAAAGAAG TTCCACCACC 1201 AAACATGCAG CTGTTACAGA CGGCCATGTA CGAACCGCCA GAAGGCTTGT GAGCCAGGAT 1261 TTTCATATAG TGAAGAAGTG TGTCGTTGTG TCCCTTCATA TTGGCAAAGA CCACAAATGA 1321 GCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1381 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1441 GGCTTTATAT ATCTTGTGGA AAGGACGAAA CGAGCTTTAA CTCGGGGGAC CGACGCGTTA 1501 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt