Construct: ORF TRCN0000468006
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015258.1_s317c1
- Derived from:
- ccsbBroadEn_09107
- DNA Barcode:
- AAACCTCACGATCATTTTAAGGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CALN1 (83698)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468006
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83698 | CALN1 | calneuron 1 | NM_001017440.3 | 99.8% | 100% | 15T>C |
| 2 | human | 83698 | CALN1 | calneuron 1 | NM_001363460.1 | 99.8% | 100% | 15T>C |
| 3 | human | 83698 | CALN1 | calneuron 1 | XM_011516596.2 | 99.8% | 100% | 15T>C |
| 4 | human | 83698 | CALN1 | calneuron 1 | XM_011516597.2 | 99.8% | 100% | 15T>C |
| 5 | human | 83698 | CALN1 | calneuron 1 | XM_011516599.1 | 99.8% | 100% | 15T>C |
| 6 | human | 83698 | CALN1 | calneuron 1 | XM_017012678.1 | 99.8% | 100% | 15T>C |
| 7 | human | 83698 | CALN1 | calneuron 1 | XM_017012679.1 | 99.8% | 100% | 15T>C |
| 8 | human | 83698 | CALN1 | calneuron 1 | XM_017012680.1 | 99.8% | 100% | 15T>C |
| 9 | human | 83698 | CALN1 | calneuron 1 | XM_017012681.1 | 99.8% | 100% | 15T>C |
| 10 | human | 83698 | CALN1 | calneuron 1 | XM_017012682.1 | 99.8% | 100% | 15T>C |
| 11 | human | 83698 | CALN1 | calneuron 1 | XM_017012683.1 | 99.8% | 100% | 15T>C |
| 12 | human | 83698 | CALN1 | calneuron 1 | XM_017012677.1 | 98.4% | 98.6% | 1_9delATGGAAAAG;24T>C |
| 13 | human | 83698 | CALN1 | calneuron 1 | XM_011516594.3 | 92.6% | 92.7% | 1_51del;66T>C |
| 14 | human | 83698 | CALN1 | calneuron 1 | NM_031468.4 | 83.7% | 83.9% | 1_126del;141T>C |
| 15 | human | 83698 | CALN1 | calneuron 1 | XM_017012675.1 | 83.7% | 83.9% | 1_126del;141T>C |
| 16 | human | 83698 | CALN1 | calneuron 1 | XM_017012676.2 | 83.7% | 83.9% | 1_126del;141T>C |
| 17 | mouse | 140904 | Caln1 | calneuron 1 | NM_021371.2 | 89.8% | 99.5% | (many diffs) |
| 18 | mouse | 140904 | Caln1 | calneuron 1 | XM_006504373.2 | 89.8% | 99.5% | (many diffs) |
| 19 | mouse | 140904 | Caln1 | calneuron 1 | XM_006504374.2 | 89.8% | 99.5% | (many diffs) |
| 20 | mouse | 140904 | Caln1 | calneuron 1 | NM_181045.1 | 75.3% | 83.5% | (many diffs) |
| 21 | mouse | 140904 | Caln1 | calneuron 1 | XM_006504372.3 | 75.3% | 83.5% | (many diffs) |
| 22 | mouse | 140904 | Caln1 | calneuron 1 | XM_006504367.2 | 72.5% | 80.4% | (many diffs) |
| 23 | mouse | 140904 | Caln1 | calneuron 1 | XM_006504368.3 | 72.5% | 80.4% | (many diffs) |
| 24 | mouse | 140904 | Caln1 | calneuron 1 | XM_006504369.2 | 72.5% | 80.4% | (many diffs) |
| 25 | mouse | 140904 | Caln1 | calneuron 1 | XM_006504366.3 | 71.7% | 79.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 723
- ORF length:
- 657
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gttccaccac gtgaccgccg gcttgttgta caaggggaat tacctcaacc 121 gatcgctctc tgctggcagt gacagcgaac agctggctaa tatctccgtg gaggagctcg 181 atgaaatccg agaggccttt cgggttctgg accgggatgg gaacggcttc atctccaagc 241 aggagctggg catggccatg cgctctttgg ggtacatgcc aagcgaggtg gagctggcca 301 tcatcatgca gcgcttggac atggacgggg atggccaggt gGATTTTGAT GAATTCATGA 361 CCATTCTTGG CCCCAAACTG GTGTCTTCAG AAGGTCGCGA TGGTTTTCTT GGGAACACGA 421 TAGACAGCAT ATTCTGGCAG TTTGACATGC AAAGGATAAC TCTGGAAGAG TTGAAGCACA 481 TTCTCTATCA TGCCTTCCGA GACCACCTAA CGATGAAGGA CATTGAGAAC ATCATTATCA 541 ATGAGGAAGA GAGCCTGAAT GAGACCTCGG GGAACTGCCA AACAGAGTTT GAAGGAGTGC 601 ATTCCCAGAA GCAGAACAGA CAGACCTGCG TCCGGAAGAG CCTCATATGC GCCTTTGCTA 661 TGGCCTTCAT CATCAGTGTC ATGCTGATTG CAGCCAACCA GATACTCCGG AGCGGCATGG 721 AGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 781 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 841 GGCTTTATAT ATCTTGTGGA AAGGACGAAA ACCTCACGAT CATTTTAAGG GTACGCGTTA 901 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt