Construct: ORF TRCN0000468018
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018105.1_s317c1
- Derived from:
- ccsbBroadEn_09866
- DNA Barcode:
- AGAGCCGGGGCCCACTGATTTCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALKBH3 (221120)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468018
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221120 | ALKBH3 | alkB homolog 3, alpha-ketog... | NM_139178.4 | 99.7% | 99.6% | 357T>C;684C>G |
| 2 | mouse | 69113 | Alkbh3 | alkB homolog 3, alpha-ketog... | NM_026944.1 | 85% | 85.3% | (many diffs) |
| 3 | mouse | 69113 | Alkbh3 | alkB homolog 3, alpha-ketog... | XM_006500123.3 | 85% | 85.3% | (many diffs) |
| 4 | mouse | 69113 | Alkbh3 | alkB homolog 3, alpha-ketog... | XM_017319248.1 | 51.3% | 53.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 927
- ORF length:
- 858
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggaaaaa agacggcgag cccgagttca gggagcctgg gctgcccctg 121 ttaaaagcca ggccattgct cagccagcta ccactgctaa gagccatctc caccagaagc 181 ctggccagac ctggaagaac aaagagcatc atctctctga cagagagttt gtgttcaaag 241 aacctcagca ggtagtacgt agagctcctg agccacgagt gattgacaga gagggtgtgt 301 atgaaatcag cctgtcaccc acaggtgtat ctagggtctg tttgtatcct ggctttgttg 361 acgtgaaaga agctgactgg atattggaac agctttgtca agatgttccc tggaaacaga 421 ggaccggcat cagagaggat ataacttatc agcaaccaag acttacagca tggtatggag 481 aacttcctta cacttattca agaaTCACTA TGGAACCAAA TCCTCACTGG CACCCTGTGC 541 TGCGCACACT AAAGAACCGC ATTGAAGAGA ACACTGGCCA CACCTTCAAC TCCTTACTCT 601 GCAATCTTTA TCGCAATGAG AAGGACAGCG TGGACTGGCA CAGTGATGAT GAACCCTCAC 661 TAGGGAGGTG CCCCATTATT GCTTCACTAA GTTTTGGTGC CACACGCACA TTTGAGATGA 721 GAAAGAAGCC ACCACCAGAA GAGAATGGAG AGTACACATA TGTGGAAAGA GTGAAGATAC 781 CCTTGGATCA TGGGACCTTG TTAATCATGG AAGGAGCGAC ACAAGCTGAC TGGCAGCATC 841 GAGTGCCCAA AGAATACCAC TCTAGAGAAC CGAGAGTGAA CCTGACCTTT CGGACAGTCT 901 ATCCAGACCC TCGAGGGGCA CCCTGGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 961 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1021 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAGAGCCGG 1081 GGCCCACTGA TTTCCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1141 aagatt