Construct: ORF TRCN0000468023
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012992.1_s317c1
- Derived from:
- ccsbBroadEn_14110
- DNA Barcode:
- CATCGGGCAGCAATTCCGGAGTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RGS17 (26575)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468023
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26575 | RGS17 | regulator of G protein sign... | NM_012419.5 | 99.8% | 2.6% | 6delA |
| 2 | human | 26575 | RGS17 | regulator of G protein sign... | XM_017010733.1 | 73.3% | 3.3% | (many diffs) |
| 3 | mouse | 56533 | Rgs17 | regulator of G-protein sign... | NM_019958.4 | 87.3% | 2.2% | (many diffs) |
| 4 | mouse | 56533 | Rgs17 | regulator of G-protein sign... | XM_006512457.3 | 87.3% | 2.2% | (many diffs) |
| 5 | mouse | 56533 | Rgs17 | regulator of G-protein sign... | NM_001161822.1 | 79.7% | 2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 192
- ORF length:
- 126
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg aaaaggcagc agtcccaaaa tgaaggaaca cctgccgtgt ctcaagctcc 121 tggaaaccag aggcccaaca acacctgttg cttttgttgg tgctgttgtt gcagctgctc 181 ctgcctcact gtgaggaatg aagaaagagg ggaaaatgcg ggaagaccca caCACACTAC 241 AAAAATGGAG AGTATCCAGG TCCTAGAGGA ATGCCAAAAC CCCACTGCAG AGGAAGTCTT 301 GTCCTGGTCT CAAAATTTTG ACAAGATGAT GAAGGCCCCA GCAGGAAGAA ACCTTTTCAG 361 AGAGTTCCTC CGAACAGAAT ACAGTGAAGA GAACCTACTT TTCTGGCTTG CTTGTGAAGA 421 CTTAAAGAAG GAGCAGAACA AAAAAGTAAT TGAAGAAAAG GCTAGGATGA TATATGAAGA 481 TTACATTTCT ATACTATCAC CAAAAGAGGT CAGTCTTGAT TCTCGAGTTA GAGAGGTGAT 541 CAATAGAAAT CTGTTGGATC CCAATCCTCA CATGTATGAA GATGCCCAAC TTCAGATATA 601 TACTTTAATG CACAGAGATT CTTTTCCAAG GTTTTTGAAC TCTCAAATTT ATAAGTCATT 661 TGTTGAAAGT ACTGCTGGCT CTTCTTCTGA ATCTTACCCA ACTTTCTTGT ACAAAGTGGT 721 TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC 781 TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA 841 CATCGGGCAG CAATTCCGGA GTTCACGCGT TAAGTCgaca atcaacctct ggattacaaa 901 atttgtgaaa gatt