Construct: ORF TRCN0000468026
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011606.1_s317c1
- Derived from:
- ccsbBroadEn_01046
- DNA Barcode:
- GCGGTGTGAGGCCTTATGTCGGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUDT1 (4521)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468026
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_198949.1 | 100% | 100% | |
| 2 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_198952.1 | 100% | 100% | |
| 3 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_198954.1 | 100% | 100% | |
| 4 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_001367553.1 | 87.1% | 87.1% | 0_1ins69 |
| 5 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_002452.3 | 87.1% | 87.1% | 0_1ins69 |
| 6 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_198948.1 | 87.1% | 87.1% | 0_1ins69 |
| 7 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_198950.1 | 87.1% | 87.1% | 0_1ins69 |
| 8 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_198953.1 | 87.1% | 87.1% | 0_1ins69 |
| 9 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_001367554.1 | 61.9% | 55.7% | (many diffs) |
| 10 | human | 4521 | NUDT1 | nudix hydrolase 1 | NM_001367555.1 | 61.9% | 55.7% | (many diffs) |
| 11 | human | 4521 | NUDT1 | nudix hydrolase 1 | NR_160264.1 | 53.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 603
- ORF length:
- 537
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tggaattagc cctcagcaga tgggggagcc agaaggcagt tggagtggga 121 agaacccagg gaccatgggc gcctccaggc tctataccct ggtgctggtc ctgcagccTC 181 AGCGAGTTCT CCTGGGCATG AAAAAGCGAG GCTTCGGGGC CGGCCGGTGG AATGGCTTTG 241 GGGGCAAAGT GCAAGAAGGA GAGACCATCG AGGATGGGGC TAGGAGGGAG CTGCAGGAGG 301 AGAGCGGTCT GACAGTGGAC GCCCTGCACA AGGTGGGCCA GATCGTGTTT GAGTTCGTGG 361 GCGAGCCTGA GCTCATGGAC GTGCATGTCT TCTGCACAGA CAGCATCCAG GGGACCCCCG 421 TGGAGAGCGA CGAAATGCGC CCATGCTGGT TCCAGCTGGA TCAGATCCCC TTCAAGGACA 481 TGTGGCCCGA CGACAGCTAC TGGTTTCCAC TCCTGCTTCA GAAGAAGAAA TTCCACGGGT 541 ACTTCAAGTT CCAGGGTCAG GACACCATCC TGGACTACAC ACTCCGCGAG GTGGACACGG 601 TCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 661 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 721 GGCTTTATAT ATCTTGTGGA AAGGACGAGC GGTGTGAGGC CTTATGTCGG ATACGCGTTA 781 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt