Construct: ORF TRCN0000468048
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005336.1_s317c1
- Derived from:
- ccsbBroadEn_13335
- DNA Barcode:
- ACCAGATCAAGGGATTTTTCTAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FNDC7 (163479)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468048
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 163479 | FNDC7 | fibronectin type III domain... | NM_001144937.3 | 68.1% | 68.2% | 1_699del;1785A>G |
| 2 | mouse | 320181 | Fndc7 | fibronectin type III domain... | NM_177091.5 | 57.4% | 58.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1569
- ORF length:
- 1500
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctttgagc gactcttcag agctgacctg cagtacaact ttcagttcct 121 gcaccatctc ttccctccag tgtggaactg aatacttgat ttcagtttta gcaagtaatg 181 atgctggatc tagcaaatca tcttcagcaa tgaccctgaa aactgttgct tgtgcacccg 241 gaagagtgac gatccaagaa gatccccctg gccacctgtc tgtggcttgg tccagtgtag 301 atctgggtga ctactatgtg gtctttgtga agagtgatga tggcttggaa gtacactgca 361 acacatctct cactcagtgc aacttcttat ctgagtgtgg cttcacttat tttattagtg 421 tttttgtcta taacaaggca gggcaaagtc ctttgggtga catattcaat tataccacag 481 ctccctgttg tcctagtgac attaaccccg tgttggtgtc cagtgacaga gttgagattg 541 tctggtctcc tgtccgtggt gccgaactgt atgaaaccaa ggctgtagat gggtacaaca 601 tggttgagtg caatgacact actcctgcgt gcaccctttc ggctctagag tgtgacacca 661 agtacaacat cacagtgtat tcattcaatg aagtccgagg cagcaatatg tcatgtactc 721 cccagttcat aaccacagct ccttgcagtc ctgaaataaa aaatgtttca agggatgcat 781 tctccatgat taatgtgcac tggcgatcca ctaatgatga tgctacttac acggtgactg 841 cccaagggga gaaaggactg tatcagtgca gcagcacagg agagtcctgc accatgcggg 901 gcttgccctg tggctcagtg ttctctgtca ctgctgtggc cgaaacacag gcaggacgga 961 gcctgcccag ctacagtgtg cccctggaaa cagtgccatg ctgtccaacc ggtctgacag 1021 taactcaaat cacccagtca gtaatcaacg tgagctggac tattgggaga gtggctcaaa 1081 cccatgttgc agttctggag tcacacactg gacagtctaa gtgtcacact catcaaaacc 1141 actgcctccT AGGGTGCATC ACATGTGGCA TCAATTACAC AGTGACATTA AAAGCAATTA 1201 GTGCCACCGG GTTGACTGCA GATTGCTCCT ACCAAAGTTA TTTCTCTGGT GCCTGCTGCC 1261 CTTTGGGGGT GAAATTATAT AGGCTGGGCC CTAATGGCAT CCGGATCTAC TGGCAAGCCT 1321 CCAGGGGCTC TGCCAATTAC AGCACTGACC TCTATGGCTC CAAAGGCATT TTCACGTGCA 1381 CCCCGAGTGC TGGCCTCAGT TTCTGTGATG TCACTGAGAT ACCCTGTGGG GATGTATACA 1441 CTGTGATGGT CTCACCAGTT GCTAAAACAG GATTGAAGCT TACTTTCTGT CCAAAAAAAA 1501 TATATTCAGT AACTTGCTCT GGAAGTACAC TTGGAATGGT GATTTATAGA GGGAAGAGAA 1561 ATGAAGAATT GCTAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1621 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1681 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACCAGA TCAAGGGATT TTTCTAAAAC 1741 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt