Construct: ORF TRCN0000468051

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007233.1_s317c1
Derived from:
ccsbBroadEn_06296
DNA Barcode:
TAGTCTGCGGCAAGACTCCTGACG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GNAZ (2781)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468051
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2781 GNAZ G protein subunit alpha z NM_002073.4 99.9% 100% 309C>T
2 human 2781 GNAZ G protein subunit alpha z XM_017028786.2 73.8% 66.3% (many diffs)
3 human 2781 GNAZ G protein subunit alpha z XR_937843.3 29% (many diffs)
4 mouse 14687 Gnaz guanine nucleotide binding ... NM_010311.3 92.1% 98.3% (many diffs)
5 mouse 14687 Gnaz guanine nucleotide binding ... XM_006513235.3 92.1% 98.3% (many diffs)
6 mouse 14687 Gnaz guanine nucleotide binding ... XM_006513236.1 92.1% 98.3% (many diffs)
7 mouse 14687 Gnaz guanine nucleotide binding ... XM_006513237.2 92.1% 98.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1131
ORF length:
1065
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg atgtcggcaa agctcagagg aaaaagaagc agcccggcgg tcccggagaa
121ttgaccgcca cctgcgctca gagagccagc ggcaacgccg cgaaatcaag ctgctcctgc
181tgggcaccag caactcaggc aagagcacca tcgtcaaaca gatgaagatc atccacagcg
241gcggcttcaa cctggaggcc tgcaaggagt acaagcccct catcatctac aatgccatcg
301actcgctgac ccgcatcatc cgggccctgg ccgccctcag gatcgacttc cacaaccccg
361accgcgccta cgatgctgtg cagctctttg cgctgacggg ccccgctgag agcaagggcg
421agatcacacc cgagctgctg ggtgtcatgc gacggctctg ggccgaccca ggggcacagg
481cctgcttcag ccgctccagc gagtaccacc tggaggacaa cgcggcctac tacctgaacg
541acctggagcg catcgccgca gctgactata tccccactgt cgaggacatc ctgcgctccc
601gggacatgac cacgggcatt gtggagaaca agttcacctt caaggagctc accttcaaga
661tggtggacgt gggggggcag aggtcagagc gcaaaaagtg gatccactgc ttcgagggcg
721tcacagccat catcttctgt gtggagctca gcggctacga cctgaaactc TACGAGGATA
781ACCAGACAAG TCGGATGGCA GAGAGCTTGC GCCTCTTTGA CTCCATCTGC AACAACAACT
841GGTTCATCAA CACCTCACTC ATCCTCTTCC TGAACAAGAA GGACCTGCTG GCAGAGAAGA
901TCCGCCGCAT CCCGCTCACC ATCTGCTTTC CCGAGTACAA GGGCCAGAAC ACGTACGAGG
961AGGCCGCTGT CTACATCCAG CGGCAGTTTG AAGACCTGAA CCGCAACAAG GAGACCAAGG
1021AGATCTACTC CCACTTCACC TGCGCCACCG ACACCAGTAA CATCCAGTTT GTCTTCGACG
1081CGGTGACAGA CGTCATCATA CAGAACAATC TCAAGTACAT TGGCCTTTGC TGCCCAACTT
1141TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1201CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1261CTTGTGGAAA GGACGATAGT CTGCGGCAAG ACTCCTGACG ACGCGTTAAG TCgacaatca
1321acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)