Construct: ORF TRCN0000468061
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001226.1_s317c1
- Derived from:
- ccsbBroadEn_00102
- DNA Barcode:
- AGATCTGAGCACTTGCCCAGGTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RND3 (390)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468061
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 390 | RND3 | Rho family GTPase 3 | NM_001254738.1 | 100% | 100% | |
| 2 | human | 390 | RND3 | Rho family GTPase 3 | NM_005168.4 | 100% | 100% | |
| 3 | mouse | 74194 | Rnd3 | Rho family GTPase 3 | NM_028810.2 | 93% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 798
- ORF length:
- 732
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggagagaaga gccagccaga aattatccag caaatctatc atggatccta 121 atcagaacgt gaaatgcaag atagttgtgg tgggagacag tcagtgtgga aaaactgcgc 181 tgctccatgt cttcgccaag gactgcttcc ccgagaatta cgttcctaca gtgtttgaga 241 attacacggc cagttttgaa atcgacacac aaagaataga gttgagcctg tgggacactt 301 cgggttctcc ttactatgac aatgtccgcc ccctctctta ccctGATTCG GATGCTGTGC 361 TGATTTGCTT TGACATCAGT AGACCAGAGA CCCTGGACAG TGTCCTCAAA AAGTGGAAAG 421 GTGAAATCCA GGAATTTTGT CCAAATACCA AAATGCTCTT GGTCGGCTGC AAGTCTGATC 481 TGCGGACAGA TGTTAGTACA TTAGTAGAGC TCTCCAATCA CAGGCAGACG CCAGTGTCCT 541 ATGACCAGGG GGCAAATATG GCCAAACAGA TTGGAGCAGC TACTTATATC GAATGCTCAG 601 CTTTACAGTC GGAAAATAGC GTCAGAGACA TTTTTCACGT TGCCACCTTG GCATGTGTAA 661 ATAAGACAAA TAAAAACGTT AAGCGGAACA AATCACAGAG AGCCACAAAG CGGATTTCAC 721 ACATGCCTAG CAGACCAGAA CTCTCGGCAG TTGCTACGGA CTTACGAAAG GACAAAGCGA 781 AGAGCTGCAC TGTGATGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 841 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 901 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGATCTG AGCACTTGCC 961 CAGGTAGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt