Construct: ORF TRCN0000468061

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001226.1_s317c1
Derived from:
ccsbBroadEn_00102
DNA Barcode:
AGATCTGAGCACTTGCCCAGGTAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RND3 (390)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468061
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 390 RND3 Rho family GTPase 3 NM_001254738.1 100% 100%
2 human 390 RND3 Rho family GTPase 3 NM_005168.4 100% 100%
3 mouse 74194 Rnd3 Rho family GTPase 3 NM_028810.2 93% 100% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
798
ORF length:
732
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa ggagagaaga gccagccaga aattatccag caaatctatc atggatccta
121atcagaacgt gaaatgcaag atagttgtgg tgggagacag tcagtgtgga aaaactgcgc
181tgctccatgt cttcgccaag gactgcttcc ccgagaatta cgttcctaca gtgtttgaga
241attacacggc cagttttgaa atcgacacac aaagaataga gttgagcctg tgggacactt
301cgggttctcc ttactatgac aatgtccgcc ccctctctta ccctGATTCG GATGCTGTGC
361TGATTTGCTT TGACATCAGT AGACCAGAGA CCCTGGACAG TGTCCTCAAA AAGTGGAAAG
421GTGAAATCCA GGAATTTTGT CCAAATACCA AAATGCTCTT GGTCGGCTGC AAGTCTGATC
481TGCGGACAGA TGTTAGTACA TTAGTAGAGC TCTCCAATCA CAGGCAGACG CCAGTGTCCT
541ATGACCAGGG GGCAAATATG GCCAAACAGA TTGGAGCAGC TACTTATATC GAATGCTCAG
601CTTTACAGTC GGAAAATAGC GTCAGAGACA TTTTTCACGT TGCCACCTTG GCATGTGTAA
661ATAAGACAAA TAAAAACGTT AAGCGGAACA AATCACAGAG AGCCACAAAG CGGATTTCAC
721ACATGCCTAG CAGACCAGAA CTCTCGGCAG TTGCTACGGA CTTACGAAAG GACAAAGCGA
781AGAGCTGCAC TGTGATGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
841TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
901GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGATCTG AGCACTTGCC
961CAGGTAGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)