Construct: ORF TRCN0000468071
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010644.1_s317c1
- Derived from:
- ccsbBroadEn_06132
- DNA Barcode:
- AATAGTCATTGGCTTTGCTCCGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DVL3 (1857)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468071
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1857 | DVL3 | dishevelled segment polarit... | NM_004423.4 | 99.9% | 99.8% | 1298G>T |
| 2 | human | 1857 | DVL3 | dishevelled segment polarit... | XM_005247172.2 | 98.6% | 98.7% | (many diffs) |
| 3 | human | 1857 | DVL3 | dishevelled segment polarit... | XM_011512513.2 | 75.2% | 72.5% | (many diffs) |
| 4 | mouse | 13544 | Dvl3 | dishevelled segment polarit... | NM_007889.2 | 90.7% | 98.8% | (many diffs) |
| 5 | mouse | 13544 | Dvl3 | dishevelled segment polarit... | XM_006521782.3 | 90.6% | 98.8% | (many diffs) |
| 6 | mouse | 13544 | Dvl3 | dishevelled segment polarit... | NM_001347176.1 | 89.5% | 97.7% | (many diffs) |
| 7 | mouse | 13544 | Dvl3 | dishevelled segment polarit... | XM_006521780.2 | 89.4% | 97.7% | (many diffs) |
| 8 | mouse | 13544 | Dvl3 | dishevelled segment polarit... | XM_011245824.2 | 58.9% | 60.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2214
- ORF length:
- 2148
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cgagaccaag atcatctacc acttggatgg gcaggagacg ccgtaccttg 121 tgaagctgcc cctgcccgcc gagcgcgtca ccttggcgga ctttaagggc gttttgcagc 181 gacccagcta taagttcttc ttcaagtcta tggacgacga tttcggagtg gtgaaggagg 241 agatctcgga tgacaatgcc aagctaccat gcttcaatgg ccgggtggtg tcctggctgg 301 tgtcagctga gggctcacac ccagacccag cccccttctg tgctgataac ccatcggagc 361 tgccaccacc tatggagcgc acgggaggca tcggggactc ccgaccccca tccttccacc 421 ctcatgctgg tgggggcagc caggagaacc tggacaatga cacagagacg gactctttgg 481 tgtctgccca gcgagagcgg ccacgccgga gggatggccc agagcatgca acccggctaa 541 atggaactgc gaagggggaa cggcggcgag aaccaggggg ttatgatagc tcatccaccc 601 ttatgagcag tgagctggag accaccagct tctttgactc agatgaggat gactccacca 661 gcaggttcag cagctccaca gaacagagca gtgcctcacg cctgatgaga agacacaagc 721 ggcggcggcg gaagcagaag gtttctcgga ttgagcggtc ctcgtccttc agcagcatca 781 cggactccac catgtcactc aacatcatca cggtcactct caacatggaa aaatataact 841 tcttgggcat ctccattgtg ggccaaagca acgagcgtgg tgacggcggc atctacattg 901 gctctatcat gaagggtggg gccgtggctg ctgatggacg catcgagcca ggagatatgt 961 tgttacaggt aaacgagatc aactttgaga acatgagtaa tgacgatgca gtccgggtac 1021 tgcgggagat tgtgcacaaa ccggggccca tcaccctgac tgtagccaag tgctgggacc 1081 caagtccacg tggttgcttc acattgccca ggagcgagcc catccggccc attgaccctg 1141 cggcctgggt ctcccacact gcagccatga ccggcacctt ccctgcatac ggcatgagcc 1201 cctccctgag caccatcacc tccaccagct cctccatcac cagttccatc cctgacacag 1261 agcgcctaga cgacttccac ttgtccatcc acagtgacat ggctgccatc gtaaaagcca 1321 tggcctcccc tgaatcaggg ttggaggtcc gtgaccgcat gttgctcaag attaccatcc 1381 ctaatgcttt catcggctca gatgtggtgg actggctgta ccacaatgtg gaaggcttca 1441 cggaccggag ggaggcccgc aagtatgcca gcaacctgct gaaagctggc ttcatccgcc 1501 ataccgtcaa caagatcacc ttctccgagc agtgctacta catcttcggt gacctctgcg 1561 gcaacatggc caacctgtct ctccacgatc acgatggctc cagtggcgcc tctgaccagg 1621 acacactggc ccctttgccg cacccggggg ccgccccttg gcccatggct ttcccgtacc 1681 agtacccgcc acccccgcac ccatacaacc cgcacccggg cttcccggag ctgggctaca 1741 gctacggcgg gggcagcgcc agcagtcagc acagcgaagg cagtcggagc agtggctcca 1801 accgtagcgg cagcgatcgg aggaaggaga aggacccgaa ggccggggac tccaagtccg 1861 ggggcagcgg cagcgaatcg gaccacacca cacgcagcag ccTGCGGGGG CCGCGGGAGC 1921 GGGCGCCCAG CGAGCGCTCA GGGCCGGCGG CCAGCGAGCA CAGCCACCGC AGCCACCATT 1981 CCCTGGCCAG CAGCCTTCGC AGCCACCACA CACACCCGAG CTACGGTCCT CCCGGAGTGC 2041 CCCCTCTCTA CGGCCCCCCC ATGCTGATGA TGCCCCCGCC GCCCGCGGCC ATGGGGCCCC 2101 CAGGAGCCCC TCCGGGCCGC GACCTGGCCT CAGTGCCCCC GGAACTGACC GCCAGCAGAC 2161 AGTCCTTCCG CATGGCCATG GGAAACCCCA GTGAGTTCTT TGTGGATGTG ATGTGCCCAA 2221 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2281 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2341 TATCTTGTGG AAAGGACGAA ATAGTCATTG GCTTTGCTCC GAAACGCGTT AAGTCgacaa 2401 tcaacctctg gattacaaaa tttgtgaaag att