Construct: ORF TRCN0000468076
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009777.1_s317c1
- Derived from:
- ccsbBroadEn_04084
- DNA Barcode:
- TGCCCGAAAGCATGAGTCCCTCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CORO7 (79585)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468076
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79585 | CORO7 | coronin 7 | NM_024535.5 | 100% | 100% | |
| 2 | human | 79585 | CORO7 | coronin 7 | NM_001201472.2 | 98% | 98% | 229_230ins54 |
| 3 | human | 79585 | CORO7 | coronin 7 | NM_001201473.2 | 90.8% | 90.8% | 229_230ins255 |
| 4 | human | 100529144 | CORO7-PAM16 | CORO7-PAM16 readthrough | NM_001201479.2 | 88.2% | 88.1% | 2774C>A;2776_3144del |
| 5 | human | 79585 | CORO7 | coronin 7 | NM_001351729.2 | 76.2% | 76.2% | 0_1ins660 |
| 6 | mouse | 78885 | Coro7 | coronin 7 | NM_030205.4 | 82.4% | 86.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2844
- ORF length:
- 2775
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaccgcttc agggtgtcca agttccggca caccgaggct cggccgcccc 121 gccgcgagtc ctggatcagt gacattcgag caggaaccgc cccttcatgc aggaaccaca 181 tcaaatcaag ctgcagcttg atcgccttca actccgaccg tcctggtgta ctgggcattg 241 tgcctctgca aggccaagga gaggacaagc gacgcgtggc ccacctgggc tgccattcag 301 acctagtcac cgacttggac ttctcgccct ttgatgactt cctcctggcc acaggctcgg 361 ctgacaggac ggtaaaactc tggcgactgc cagggcctgg ccaggccctg ccctcagcac 421 ccggggtggt gctgggcccc gaggacctcc cagtggaggt actgcagttc caccccacct 481 ctgacggcat tctggtgagc gcagcaggca ccactgtgaa ggtctgggac gcagccaagc 541 agcagcccct gacagagctg gcagcccatg gggacctggt gcagagcgcc gtctggagcc 601 gagatggagc cctggtgggc acggcgtgca aggacaagca gctgcggatc tttgacccca 661 gaacaaagcc gcgggcctct cagagcacgc aggcccatga gaacagcagg gatagccggc 721 tggcatggat gggcacctgg gagcaccttg tgtctactgg attcaaccag atgcgtgagc 781 gcgaagtgaa gctgtgggac acgcggttct tctccagcgc cctggcctcc ctcaccttgg 841 acacctcgct tgggtgtctc gtgcctctgc tggaccctga ctctgggctc ctggtcctgg 901 caggaaaggg cgagaggcag ctgtactgtt acgaggtggt cccgcagcag ccggcgctga 961 gcccagtgac ccagtgtgtc ctggagagcg tgctgcgtgg ggctgccctt gtgccccggc 1021 aggcgctggc cgtcatgagc tgcgaggtac tccgcgtcct acagctgagc gacacagcca 1081 tcgtgcccat cggctaccat gtgccccgca aggctgtgga gttccacgag gacctgttcc 1141 cggacactgc cggctgtgtg cctgccaccg acccccatag ctggtgggct ggggacaacc 1201 agcaggtgca gaaggtcagc ctcaaccccg cctgccggcc ccacccgagc ttcacttcct 1261 gtctggtgcc ccctgcggag cccctccctg acacagccca gcctgcggtg atggagacac 1321 ccgtgggtga tgcagacgca agcgagggtt tctcttcccc tcccagttcg ctgacctcgc 1381 cctccacgcc ctccagcctg gggccctcac tctccagcac cagtggcatc gggaccagcc 1441 ccagtttgag gtcgctgcag agcctgctgg gccccagttc caagttccgc catgctcagg 1501 gcactgtcct gcaccgagac agccacatca ccaacctcaa ggggctcaac ctcaccacac 1561 ctggtgagag tgacggcttc tgtgccaaca agctgcgtgt ggccgtgccg ctgctcagca 1621 gcgggggaca ggtggctgtg cttgagctac ggaagcctgg ccgcctgccc gacacggcac 1681 tgcccacgct gcagaatggg gcagctgtga ctgatctggc ctgggacccc tttgaccccc 1741 atcgcctcgc tgtggctggt gaggacgcca ggatccgact gtggcgggta cccgcagagg 1801 gcctggaaga ggtgctcacc acgccagaga ctgtgctcac aggccacacg gagaagatct 1861 gctccctgcg cttccaccca ctggcagcca atgtgctggc ctcgtcctcc tatgacctca 1921 ctgttcgcat ctgggacctt caggctggag ctgatcggct gaagctgcag ggccaccaag 1981 accagatctt cagcctggcc tggagtcctg atgggcagca gctggccact gtctgcaagg 2041 atgggcgtgt gcgggtctac aggccccgga gtggccctga gcccctgcag gaaggcccag 2101 ggcccaaggg aggacgcgga gctcgcattg tctgggtatg tgatggtcgc tgtctgctgg 2161 tgtctggctt tgacagccaa agtgagcgcc agctgctcct atatgaagct gaggccctgg 2221 ccggcggacc cttggcagtg ttgggcctgg acgtggctcc ctcaaccctg ctgcccagct 2281 acgacccaga cactggcctg gtgctcctga ccggcaaggg cgacacccgt gtattcctgt 2341 acgagctgct ccccgagtcc cctttcttcc tggagtgcaa cagcttcacg tcgcctgacc 2401 cccacaaggg cctcgtcctc ctgccTAAGA CGGAGTGCGA CGTGCGGGAA GTGGAGCTGA 2461 TGCGGTGCCT GCGGCTGCGT CAGTCCTCCC TGGAGCCTGT GGCCTTCCGG CTGCCCCGAG 2521 TCCGGAAAGA GTTCTTCCAG GATGACGTGT TCCCAGACAC GGCTGTGATC TGGGAGCCTG 2581 TGCTCAGTGC CGAGGCCTGG CTGCAAGGCG CTAATGGGCA GCCCTGGCTT CTCAGCCTGC 2641 AGCCTCCTGA CATGAGCCCA GTGAGCCAAG CCCCCCGAGA GGCCCCTGCT CGTCGGGCCC 2701 CATCCTCAGC GCAGTACCTG GAAGAAAAGT CTGACCAGCA AAAGAAGGAG GAGCTGCTGA 2761 ATGCCATGGT GGCAAAACTG GGGAACCGGG AGGACCCACT CCCCCAGGAC TCCTTTGAAG 2821 GCGTGGACGA GGACGAGTGG GACTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2881 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2941 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GCCCGAAAGC 3001 ATGAGTCCCT CTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 3061 att