Construct: ORF TRCN0000468078
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012300.1_s317c1
- Derived from:
- ccsbBroadEn_04383
- DNA Barcode:
- AGGTCGGTCCTAGCCACCAGCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCGF5 (84333)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468078
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84333 | PCGF5 | polycomb group ring finger 5 | NM_001256549.1 | 100% | 100% | |
| 2 | human | 84333 | PCGF5 | polycomb group ring finger 5 | NM_001257101.1 | 100% | 100% | |
| 3 | human | 84333 | PCGF5 | polycomb group ring finger 5 | NM_032373.5 | 100% | 100% | |
| 4 | human | 84333 | PCGF5 | polycomb group ring finger 5 | XM_011540271.3 | 100% | 100% | |
| 5 | human | 84333 | PCGF5 | polycomb group ring finger 5 | XM_017016776.2 | 100% | 100% | |
| 6 | human | 84333 | PCGF5 | polycomb group ring finger 5 | XM_011540272.2 | 92.1% | 92.1% | 663_664ins60 |
| 7 | human | 84333 | PCGF5 | polycomb group ring finger 5 | XM_017016777.2 | 92.1% | 92.1% | 663_664ins60 |
| 8 | human | 84333 | PCGF5 | polycomb group ring finger 5 | XM_006718030.1 | 74.6% | 72.6% | 0_1ins98;14_15ins97 |
| 9 | human | 84333 | PCGF5 | polycomb group ring finger 5 | XM_017016778.1 | 66.7% | 64.8% | 0_1ins98;14_15ins97;468_469ins60 |
| 10 | mouse | 76073 | Pcgf5 | polycomb group ring finger 5 | XM_006527431.3 | 90.3% | 96% | (many diffs) |
| 11 | mouse | 76073 | Pcgf5 | polycomb group ring finger 5 | XM_011247391.2 | 90.3% | 96% | (many diffs) |
| 12 | mouse | 76073 | Pcgf5 | polycomb group ring finger 5 | XM_011247392.2 | 90.3% | 96% | (many diffs) |
| 13 | mouse | 76073 | Pcgf5 | polycomb group ring finger 5 | NM_029508.3 | 83.3% | 89.4% | (many diffs) |
| 14 | mouse | 76073 | Pcgf5 | polycomb group ring finger 5 | XM_006527433.3 | 83.3% | 89.4% | (many diffs) |
| 15 | mouse | 76073 | Pcgf5 | polycomb group ring finger 5 | XM_011247395.2 | 83.3% | 89.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 834
- ORF length:
- 768
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tacccaaagg aaacacttgg tgaaagattt taatccttac attacctgct 121 atatctgtaa agggtatctg atcaagccaa caacagtgac ggaatgcctc catacattct 181 gtaagacttg tattgttcag cactttgaag atagcaatga ttgcccaagg tgtggcaacc 241 aagttcatga gacaaatcca ttagaaatgt tgaggttgga caatacatta gaggaaatta 301 tatttaagct ggtccctgga ctacgagaac aagaacttga gcgtgaatct gaattttGGA 361 AGAAAAATAA GCCTCAAGAA AATGGACAAG ATGATACTTC AAAAGCTGAC AAACCGAAAG 421 TAGATGAAGA AGGTGATGAA AATGAAGATG ATAAAGATTA TCACAGAAGT GACCCACAAA 481 TTGCTATCTG TCTAGATTGT TTACGAAATA ATGGGCAATC AGGGGACAAT GTAGTAAAGG 541 GTTTAATGAA GAAATTCATT CGATGTTCTA CACGTGTAAC TGTGGGAACT ATTAAAAAAT 601 TTCTAAGTTT AAAACTAAAA CTTCCAAGTT CTTATGAGTT GGATGTGCTG TGCAATGGTG 661 AAATTATGGG GAAGGATCAT ACTATGGAAT TCATCTACAT GACAAGATGG CGACTAAGAG 721 GCGAAAACTT TCGGTGTCTG AACTGCTCAG CTTCGCAAGT CTGCTCTCAG GATGGCCCTT 781 TGTATCAGTC ATACCCTATG GTACTGCAGT ATCGACCAAG AATTGATTTC GGTTACCCAA 841 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 901 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 961 TATCTTGTGG AAAGGACGAA GGTCGGTCCT AGCCACCAGC TCAACGCGTT AAGTCgacaa 1021 tcaacctctg gattacaaaa tttgtgaaag att