Construct: ORF TRCN0000468099
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011397.1_s317c1
- Derived from:
- ccsbBroadEn_01381
- DNA Barcode:
- CTCTGACTTTCCCACCGTGGAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM3 (5935)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468099
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5935 | RBM3 | RNA binding motif protein 3 | NM_006743.5 | 100% | 100% | |
| 2 | human | 5935 | RBM3 | RNA binding motif protein 3 | XM_024452413.1 | 55.9% | 5.2% | (many diffs) |
| 3 | human | 5935 | RBM3 | RNA binding motif protein 3 | XM_024452414.1 | 55.9% | 5.2% | (many diffs) |
| 4 | mouse | 19652 | Rbm3 | RNA binding motif (RNP1, RR... | NM_001166409.2 | 87.2% | 94.9% | (many diffs) |
| 5 | mouse | 19652 | Rbm3 | RNA binding motif (RNP1, RR... | NM_001293658.1 | 87.2% | 94.9% | (many diffs) |
| 6 | mouse | 19652 | Rbm3 | RNA binding motif (RNP1, RR... | NM_016809.6 | 87.2% | 94.9% | (many diffs) |
| 7 | mouse | 19652 | Rbm3 | RNA binding motif (RNP1, RR... | NM_001166410.2 | 86.8% | 94.2% | (many diffs) |
| 8 | mouse | 19652 | Rbm3 | RNA binding motif (RNP1, RR... | NM_001166411.2 | 86.8% | 94.2% | (many diffs) |
| 9 | mouse | 100043257 | Rbm3-ps | RNA binding motif (RNP1, RR... | XR_878599.2 | 35.5% | (many diffs) | |
| 10 | mouse | 19652 | Rbm3 | RNA binding motif (RNP1, RR... | XR_001782742.1 | 26% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 537
- ORF length:
- 471
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaac 61 ttggcatgtc ctctgaagaa ggaaagctct tcgtgggagg gctcaacttt aacaccgacg 121 agcaggcact ggaagaccac ttcagcagtt tcggacctat ctctgaggtg gtcgttgtca 181 aggaccggga gactcagcgg tccaggggtt ttggtttcat caccttcacc aacccagagc 241 atgcttcagt tgccatgaga gccatgaacg gagagtcTCT GGATGGTCGT CAGATCCGTG 301 TGGATCATGC AGGCAAGTCT GCTCGGGGAA CCAGAGGAGG TGGCTTTGGG GCCCATGGGC 361 GTGGTCGCAG CTACTCTAGA GGTGGTGGGG ACCAGGGCTA TGGGAGTGGC AGGTATTATG 421 ACAGTCGACC TGGAGGGTAT GGATATGGAT ATGGACGTTC CAGAGACTAT AATGGCAGAA 481 ACCAGGGTGG TTATGACCGC TACTCAGGAG GAAATTACAG AGACAATTAT GACAACTGCC 541 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 601 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 661 ATATATCTTG TGGAAAGGAC GACTCTGACT TTCCCACCGT GGAATTACGC GTTAAGTCga 721 caatcaacct ctggattaca aaatttgtga aagatt