Construct: ORF TRCN0000468127
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010433.1_s317c1
- Derived from:
- ccsbBroadEn_09736
- DNA Barcode:
- GGCATTTACGATATTGCCTGAGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAAF4 (161582)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468127
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 161582 | DNAAF4 | dynein axonemal assembly fa... | NM_001033560.1 | 99.8% | 99.4% | 572A>G;995C>T |
| 2 | human | 161582 | DNAAF4 | dynein axonemal assembly fa... | NM_001033559.2 | 92.6% | 91.4% | (many diffs) |
| 3 | human | 161582 | DNAAF4 | dynein axonemal assembly fa... | NM_130810.4 | 86% | 84.3% | (many diffs) |
| 4 | human | 100533483 | DNAAF4-CCPG1 | DNAAF4-CCPG1 readthrough (N... | NR_037923.1 | 22.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1209
- ORF length:
- 1143
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tcttcaggtt agcgattaca gctggcagca gacgaagact gcggtctttc 121 tgtctctgcc cctcaaaggc gtgtgcgtca gagacacgga cgtgttctgc acggaaaact 181 atctgaaggt caactttcct ccatttttat ttgaggcatt tctttatgct cccatagacg 241 atgagagcag caaagcaaag attgggaatg acaccattgt cttcaccttg tataaaaaag 301 aagcggccat gtgggagacc ctttctgtga cgggtgttga caaagagatg atgcaaagaa 361 ttagagaaaa atctatttta caagcacaag agagagcaaa agaagctaca gaagcaaaag 421 ctgcagcaaa gcgggaagat caaaaatacg cactaagtgt catgatgaag attgaagaag 481 aagagaggaa aaaaatagaa gatatgaaag aaaatgaacg gataaaagcc actaaagcat 541 tggaagcctg gaaagaatat caaagaaaag ctgaggagca aaaaaaaatt cagagagaag 601 agaaattatg tcaaaaagaa aagcaaatta aagaaggaag aaaaaaaata aaatataaga 661 gtcttactag aaatttggca tctagaaatc ttgctccaaa agggagaaat tcagaaaata 721 tatttactga gaagttaaag gaagacagta ttcctgctcc tcgctctgtt ggcagtatta 781 aaatcaactt tacccctcga gtattcccaa cagctcttcg tgaaTCACAA GTAGCAGAAG 841 AGGAGGAGTG GCTACACAAA CAAGCTGAGG CACGAAGAGC AATGAATACT GACATAGCTG 901 AACTTTGCGA TTTAAAAGAA GAAGAAAAGA ACCCAGAATG GTTGAAGGAT AAAGGAAACA 961 AATTGTTTGC AACGGAAAAC TATTTGGCAG CTATCAATGC ATATAATTTA GCCATAAGAC 1021 TAAATAATAA GATGCCACTA TTGTATTTGA ACCGGGCTGT TTGCCACCTA AAACTAAAAA 1081 ACTTACACAA GGCTATTGAA GATTCTTCTA AGGAATTCTG TTCTCTTGAG GGCATTGAAT 1141 GTCAGGCCTC TGAGCCCAAG CTAAGCCATC ATATCCCTAG TGACCTGCAC GTATACATCC 1201 AGATGGCCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1261 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1321 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGGCATT TACGATATTG CCTGAGTTAC 1381 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt