Construct: ORF TRCN0000468128
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013910.1_s317c1
- Derived from:
- ccsbBroadEn_03771
- DNA Barcode:
- GTATACTTGTCGACCCACTTTTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COQ9 (57017)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468128
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57017 | COQ9 | coenzyme Q9 | NM_020312.4 | 100% | 100% | |
| 2 | mouse | 67914 | Coq9 | coenzyme Q9 | NM_026452.2 | 87.9% | 90.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1020
- ORF length:
- 954
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggcggcg gtatctggtg cgcttggccg ggcgggctgg aggctcctgc 121 agctgcgatg cctgcccgtg gcccgttgcc gacaagccct ggtgccgcgt gccttccatg 181 cttcagctgt ggggctaagg tcttcagatg agcagaagca gcagcctccc aactcatttt 241 ctcagcagca ttctgagaca cagggggcag aaaaacctga tccagagtct tctcattcac 301 cccccaggta tacagaccag ggcggcgagg aggaggagga ctatgaaagt gaggagcagt 361 tgcagcaccg catcctgacg gcagcccttg agtttgtgcc cgcccacggg tggacagcag 421 aggcgattgc agaaggagcc cagtctctgg gtctctccag tgcagcagcc agcatgttcg 481 ggaaggatgg cagtgagcta atactgcatt ttgtgaccca gtgcaatacc cggctcacac 541 gtgtgctaga agaggagcag aagctggtac agttgggcca ggcggagaag aggaagacag 601 accagttccT GAGGGATGCA GTGGAAACCA GACTGAGAAT GCTGATCCCA TACATTGAGC 661 ACTGGCCCCG GGCCCTCAGC ATCCTCATGC TCCCTCACAA CATCCCGTCC AGCCTGAGCC 721 TGCTCACCAG CATGGTGGAT GACATGTGGC ATTACGCTGG GGACCAGTCC ACTGATTTTA 781 ACTGGTACAC CCGCCGAGCC ATGCTGGCTG CCATCTACAA CACAACAGAG CTGGTGATGA 841 TGCAGGACTC CTCTCCAGAC TTTGAGGACA CTTGGCGCTT CCTGGAAAAC CGGGTTAATG 901 ATGCAATGAA CATGGGCCAC ACTGCCAAGC AGGTAAAGTC CACAGGAGAG GCACTGGTGC 961 AAGGACTCAT GGGTGCAGCA GTGACGCTCA AGAACTTGAC AGGTCTAAAC CAGCGTCGGT 1021 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1081 GTCTCGATTC TACGTAGTAA TGAACTAGCC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1141 TTTATATATC TTGTGGAAAG GACGAGTATA CTTGTCGACC CACTTTTAGA CGCGTTAAGT 1201 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt