Construct: ORF TRCN0000468130
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001807.1_s317c1
- Derived from:
- ccsbBroadEn_08428
- DNA Barcode:
- CCCGTAAACACTCCTTGTCTCGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ELOVL2 (54898)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468130
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54898 | ELOVL2 | ELOVL fatty acid elongase 2 | NM_017770.4 | 99.7% | 99.6% | 234C>A;646A>G |
| 2 | human | 54898 | ELOVL2 | ELOVL fatty acid elongase 2 | XM_011514717.3 | 99.4% | 99.3% | 1_3delATG;237C>A;649A>G |
| 3 | human | 54898 | ELOVL2 | ELOVL fatty acid elongase 2 | XM_011514716.3 | 90.4% | 90.1% | 1_91delinsA;324C>A;736A>G |
| 4 | human | 54898 | ELOVL2 | ELOVL fatty acid elongase 2 | XM_017010985.1 | 35.8% | 34.6% | (many diffs) |
| 5 | mouse | 54326 | Elovl2 | elongation of very long cha... | NM_019423.2 | 84.2% | 88.1% | (many diffs) |
| 6 | mouse | 54326 | Elovl2 | elongation of very long cha... | NM_001311121.1 | 79.3% | 83.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 954
- ORF length:
- 888
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga acatctaaag gcctttgatg atgaaatcaa tgcttttttg gacaatatgt 121 ttggaccgcg agattctcga gtcagagggt ggttcatgtt ggactcttac cttcctacct 181 tttttcttac tgtcatgtat ctgctctcaa tatggctggg taacaagtat atgaagaaca 241 gacctgctct ttctctcagg ggtatcctca ccttgtataa tcttggaatc acacttctat 301 ccgcgtacat gctggcagag ctcattctct ccacttggga aggaggctac aacttacagt 361 gtcaagatct taccagcgca ggggaagctg acatccgggt agccaaggtg ctttggtggt 421 actatttctc caaatcagta gagttcctgg acacaatttt cttcgttttg cggaaaaaaa 481 cgagTCAGAT TACTTTTCTT CATGTATATC ATCATGCTTC TATGTTTAAC ATCTGGTGGT 541 GTGTCTTGAA CTGGATACCT TGTGGACAAA GTTTCTTTGG ACCAACACTG AACAGTTTTA 601 TCCACATTCT TATGTACTCC TACTATGGAC TTTCTGTGTT TCCATCTATG CACAAGTATC 661 TTTGGTGGAA GAAATATCTC ACACAGGCTC AGCTGGTGCA GTTCGTGCTC GCCATCACGC 721 ACACCATGAG CGCCGTCGTG AAACCGTGTG GCTTCCCCTT CGGTTGTCTC ATCTTCCAGT 781 CATCTTATAT GCTAACGTTA GTCATCCTCT TCTTAAATTT TTACGTTCAG ACATACCGAA 841 AAAAGCCAAT GAAGAAAGAT ATGCAAGAGC CACCTGCAGG GAAAGAAGTG AAGAATGGTT 901 TTTCCAAAGC CTACTTCACT GCAGCAAATG GAGTGATGAA CAAGAAAGCA CAATACCCAA 961 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1021 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1081 TATCTTGTGG AAAGGACGAC CCGTAAACAC TCCTTGTCTC GATACGCGTT AAGTCgacaa 1141 tcaacctctg gattacaaaa tttgtgaaag att