Construct: ORF TRCN0000468134

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009342.1_s317c1
Derived from:
ccsbBroadEn_08305
DNA Barcode:
CGGGTGGCTTCCCGTGTACTAGGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DTL (51514)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468134
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51514 DTL denticleless E3 ubiquitin p... NM_016448.4 99.9% 99.7% 1307C>T;2081A>C
2 human 51514 DTL denticleless E3 ubiquitin p... NM_001286230.1 94.2% 94.2% 50_51ins126
3 human 51514 DTL denticleless E3 ubiquitin p... XM_011509614.1 91.4% 91.2% 0_1ins186;1121C>T;1895A>C
4 human 51514 DTL denticleless E3 ubiquitin p... NM_001286229.1 62.7% 62.7% 1_1delAins814;3G>T
5 mouse 76843 Dtl denticleless E3 ubiquitin p... NM_029766.3 88% 89.4% (many diffs)
6 mouse 76843 Dtl denticleless E3 ubiquitin p... NM_001305233.1 70.6% 70% (many diffs)
7 mouse 76843 Dtl denticleless E3 ubiquitin p... XM_011238925.1 68.2% 69.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2256
ORF length:
2190
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgct cttcaattcg gtgctccgcc agccccagct tggcgtcctg agaaatggat
121ggtcttcaca ataccctctt caatcccttc tgactggtta tcagtgcagt ggtaatgatg
181aacacacttc ttatggagaa acaggagtcc cagttcctcc ttttggatgt accttctctt
241ctgctcccaa tatggaacat gtactagcag ttgccaatga agaaggcttt gttcgattgt
301ataacacaga atcacaaagt ttcagaaaga agtgcttcaa agaatggatg gctcactgga
361atgccgtctt tgacctggcc tgggttcctg gtgaacttaa acttgttaca gcagcaggtg
421atcaaacagc caaattttgg gacgtaaaag ctggtgagct gattggaaca tgcaaaggtc
481atcaatgcag cctcaagtca gttgcctttt ctaagtttga gaaagctgta ttctgtacgg
541gtggaagaga tggcaacatt atggtctggg ataccaggtg caacaaaaaa gatgggtttt
601ataggcaagt gaatcaaatc agtggagctc acaatacctc agacaagcaa accccttcaa
661aacccaagaa gaaacagaat tcaaaaggac ttgctccttc tgtggatttc cagcaaagtg
721ttactgtggt cctctttcaa gacgagaata ccttagtctc agcaggagct gtggatggga
781taatcaaagt atgggattta cgtaagaatt atactgctta tcgacaagaa cccatagcat
841ccaagtcttt cctgtaccca ggtagcagca ctcgaaaact tggatattca agtctgattt
901tggattccac tggctctact ttatttgcta attgcacaga cgataacatc tacatgttta
961atatgactgg gttgaagact tctccagtgg ctattttcaa tggacaccag aactctacct
1021tttatgtaaa atccagcctt agtccagatg accagttttt agtcagtggc tcaagtgatg
1081aagctgccta catatggaag gtctccacac cctggcaacc tcctactgtg ctcctgggtc
1141attctcaaga ggtcacgtct gtgtgctggt gtccatctga cttcacaaag attgctacct
1201gttctgatga caatacacta aaaatctggc gcttgaatag aggcttagag gagaaaccag
1261gaggtgataa actttccacg gtgggttggg cctctcagaa gaaaaaagag tcaagacctg
1321gcctagtaac agtaacgagt agccagagta ctcctgccaa agcccccagg gtaaagtgca
1381atccatccaa ttcttccccg tcatccgcag cttgtgcccc aagctgtgct ggagacctcc
1441ctcttccttc aaatactcct acgttctcta ttaaaacctc tcctgccaag gcccggtctc
1501ccatcaacag aagaggctct gtctcctccg tctctcccaa gccaccttca tctttcaaga
1561tgtcgattag aaactgggtg acccgaacac cttcctcatc accacccatc actccacctg
1621cttcggagac caagatcatg tctccgagaa aagcccttat tcctgtgagc cagaagtcat
1681cccaagcaga ggcttgctct gagtctagaa atagagtaaa gaggaggcta gactcaagct
1741gtctggagag tgtgaaacaa aagtgtgtga agagttgtaa ctgtgtgact gagcttgatg
1801gccaagttga aaatcttcat ttggatctgt gctgccttgc tggtaaccag gaagacctta
1861gtaaggactc tctaggtcct accaaatcaa gcaaaattga aggagctggt accagtatct
1921cagagccTCC GTCTCCTATC AGTCCGTATG CTTCAGAAAG CTGTGGAACG CTACCTCTTC
1981CTTTGAGACC TTGTGGAGAA GGGTCTGAAA TGGTAGGCAA AGAGAATAGT TCCCCAGAGA
2041ATAAAAACTG GTTGTTGGCC ATGGCAGCCA AACGGAAGGC TGAGAATCCA TCTCCACGAA
2101GTCCGTCATC CCAGACACCC AATTCCAGGA GACAGAGCGG AAAGACATTG CCAAGCCCGG
2161TCACCATCAC GCCCAGCTCC ATGAGGAAAA TCTGCACATA CTTCCATAGA AAGTCCCAGG
2221AGGACTTCTG TGGTCCTGAA CACTCAACAG AATTATACCC AACTTTCTTG TACAAAGTGG
2281TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
2341CTAGTCCGTA ACTTGAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA
2401CGGGTGGCTT CCCGTGTACT AGGCACGCGT TAAGTCgaca atcaacctct ggattacaaa
2461atttgtgaaa gatt

Download FASTA (ORF) (Full)