Construct: ORF TRCN0000468134
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009342.1_s317c1
- Derived from:
- ccsbBroadEn_08305
- DNA Barcode:
- CGGGTGGCTTCCCGTGTACTAGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DTL (51514)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468134
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51514 | DTL | denticleless E3 ubiquitin p... | NM_016448.4 | 99.9% | 99.7% | 1307C>T;2081A>C |
| 2 | human | 51514 | DTL | denticleless E3 ubiquitin p... | NM_001286230.1 | 94.2% | 94.2% | 50_51ins126 |
| 3 | human | 51514 | DTL | denticleless E3 ubiquitin p... | XM_011509614.1 | 91.4% | 91.2% | 0_1ins186;1121C>T;1895A>C |
| 4 | human | 51514 | DTL | denticleless E3 ubiquitin p... | NM_001286229.1 | 62.7% | 62.7% | 1_1delAins814;3G>T |
| 5 | mouse | 76843 | Dtl | denticleless E3 ubiquitin p... | NM_029766.3 | 88% | 89.4% | (many diffs) |
| 6 | mouse | 76843 | Dtl | denticleless E3 ubiquitin p... | NM_001305233.1 | 70.6% | 70% | (many diffs) |
| 7 | mouse | 76843 | Dtl | denticleless E3 ubiquitin p... | XM_011238925.1 | 68.2% | 69.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2256
- ORF length:
- 2190
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct cttcaattcg gtgctccgcc agccccagct tggcgtcctg agaaatggat 121 ggtcttcaca ataccctctt caatcccttc tgactggtta tcagtgcagt ggtaatgatg 181 aacacacttc ttatggagaa acaggagtcc cagttcctcc ttttggatgt accttctctt 241 ctgctcccaa tatggaacat gtactagcag ttgccaatga agaaggcttt gttcgattgt 301 ataacacaga atcacaaagt ttcagaaaga agtgcttcaa agaatggatg gctcactgga 361 atgccgtctt tgacctggcc tgggttcctg gtgaacttaa acttgttaca gcagcaggtg 421 atcaaacagc caaattttgg gacgtaaaag ctggtgagct gattggaaca tgcaaaggtc 481 atcaatgcag cctcaagtca gttgcctttt ctaagtttga gaaagctgta ttctgtacgg 541 gtggaagaga tggcaacatt atggtctggg ataccaggtg caacaaaaaa gatgggtttt 601 ataggcaagt gaatcaaatc agtggagctc acaatacctc agacaagcaa accccttcaa 661 aacccaagaa gaaacagaat tcaaaaggac ttgctccttc tgtggatttc cagcaaagtg 721 ttactgtggt cctctttcaa gacgagaata ccttagtctc agcaggagct gtggatggga 781 taatcaaagt atgggattta cgtaagaatt atactgctta tcgacaagaa cccatagcat 841 ccaagtcttt cctgtaccca ggtagcagca ctcgaaaact tggatattca agtctgattt 901 tggattccac tggctctact ttatttgcta attgcacaga cgataacatc tacatgttta 961 atatgactgg gttgaagact tctccagtgg ctattttcaa tggacaccag aactctacct 1021 tttatgtaaa atccagcctt agtccagatg accagttttt agtcagtggc tcaagtgatg 1081 aagctgccta catatggaag gtctccacac cctggcaacc tcctactgtg ctcctgggtc 1141 attctcaaga ggtcacgtct gtgtgctggt gtccatctga cttcacaaag attgctacct 1201 gttctgatga caatacacta aaaatctggc gcttgaatag aggcttagag gagaaaccag 1261 gaggtgataa actttccacg gtgggttggg cctctcagaa gaaaaaagag tcaagacctg 1321 gcctagtaac agtaacgagt agccagagta ctcctgccaa agcccccagg gtaaagtgca 1381 atccatccaa ttcttccccg tcatccgcag cttgtgcccc aagctgtgct ggagacctcc 1441 ctcttccttc aaatactcct acgttctcta ttaaaacctc tcctgccaag gcccggtctc 1501 ccatcaacag aagaggctct gtctcctccg tctctcccaa gccaccttca tctttcaaga 1561 tgtcgattag aaactgggtg acccgaacac cttcctcatc accacccatc actccacctg 1621 cttcggagac caagatcatg tctccgagaa aagcccttat tcctgtgagc cagaagtcat 1681 cccaagcaga ggcttgctct gagtctagaa atagagtaaa gaggaggcta gactcaagct 1741 gtctggagag tgtgaaacaa aagtgtgtga agagttgtaa ctgtgtgact gagcttgatg 1801 gccaagttga aaatcttcat ttggatctgt gctgccttgc tggtaaccag gaagacctta 1861 gtaaggactc tctaggtcct accaaatcaa gcaaaattga aggagctggt accagtatct 1921 cagagccTCC GTCTCCTATC AGTCCGTATG CTTCAGAAAG CTGTGGAACG CTACCTCTTC 1981 CTTTGAGACC TTGTGGAGAA GGGTCTGAAA TGGTAGGCAA AGAGAATAGT TCCCCAGAGA 2041 ATAAAAACTG GTTGTTGGCC ATGGCAGCCA AACGGAAGGC TGAGAATCCA TCTCCACGAA 2101 GTCCGTCATC CCAGACACCC AATTCCAGGA GACAGAGCGG AAAGACATTG CCAAGCCCGG 2161 TCACCATCAC GCCCAGCTCC ATGAGGAAAA TCTGCACATA CTTCCATAGA AAGTCCCAGG 2221 AGGACTTCTG TGGTCCTGAA CACTCAACAG AATTATACCC AACTTTCTTG TACAAAGTGG 2281 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2341 CTAGTCCGTA ACTTGAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA 2401 CGGGTGGCTT CCCGTGTACT AGGCACGCGT TAAGTCgaca atcaacctct ggattacaaa 2461 atttgtgaaa gatt