Construct: ORF TRCN0000468135
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011691.1_s317c1
- Derived from:
- ccsbBroadEn_10948
- DNA Barcode:
- AATTATATACCGAAGTCCCTCCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FADS1 (3992)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468135
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3992 | FADS1 | fatty acid desaturase 1 | XM_011545022.2 | 88.7% | 84% | (many diffs) |
| 2 | human | 3992 | FADS1 | fatty acid desaturase 1 | NM_013402.7 | 88.5% | 88.4% | 1_171del;985C>T |
| 3 | mouse | 76267 | Fads1 | fatty acid desaturase 1 | NM_146094.2 | 85.1% | 86.8% | (many diffs) |
| 4 | mouse | 76267 | Fads1 | fatty acid desaturase 1 | XM_006527438.1 | 62.6% | 65% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1398
- ORF length:
- 1332
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccccgacccg gtggccgccg agaccgcggc tcagggacct accccgcgct 121 acttcacctg ggacgaggtg gcccagcgct cagggtgcga ggagcggtgg ctagtgatcg 181 accgtaaggt gtacaacatc agcgagttca cccgccggca tccagggggc tcccgggtca 241 tcagccacta cgccgggcag gatgccacgg atccctttgt ggccttccac atcaacaagg 301 gccttgtgaa gaagtatatg aactctctcc tgattggaga actgtctcca gagcagccca 361 gctttgagcc caccaagaat aaagagctga cagatgagtt ccgggagctg cgggccacag 421 tggagcggat ggggctcatg aaggccaacc atgtcttctt cctgctgtac ctgctgcaca 481 tcttgctgct ggatggtgca gcctggctca ccctttgggt ctttgggacg tcctttttgc 541 ccttcctcct ctgtgcggtg ctgctcagtg cagttcaggc ccaggctggc tggctgcagc 601 atgactttgg gcacctgtcg gtcttcagca cctcaaagtg gaaccatctg ctacatcatt 661 ttgtgattgg ccacctgaag ggggcccccg ccagttggtg gaaccacatg cacttccagc 721 accatgccaa gcccaactgc ttccgcaaag acccagacat caacatgcat cccttcttct 781 ttgccttggg gaagatcctc tctgtggagc ttgggaaaca gaagaaaaaa tatatgccgt 841 acaaccacca gcacaaatac ttcttcctaa ttgggccctc agccttgctg cctctctact 901 tccagtggta tattttctat tttgttatcc agcgaaagaa gtgggtggac ttggcctgga 961 tgattacctt ctacgtccgc ttcttcctca cttatgtgcc actattgggg ctgaaagcct 1021 tcctgggCCT TTTCTTCATA GTCAGGTTCC TGGAAAGCAA CTGGTTTGTG TGGGTGACAC 1081 AGATGAACCA TATTCCCATG CACATTGATC ATGACCGGAA CATGGACTGG GTTTCCACCC 1141 AGCTCCAGGC CACATGCAAT GTCCACAAGT CTGCCTTCAA TGACTGGTTC AGTGGACACC 1201 TCAACTTCCA GATTGAGCAC CATCTTTTTC CCACGATGCC TCGACACAAT TACCACAAAG 1261 TGGCTCCCCT GGTGCAGTCC TTGTGTGCCA AGCATGGCAT AGAGTACCAG TCCAAGCCCC 1321 TGCTGTCAGC CTTCGCCGAC ATCATCCACT CACTAAAGGA GTCAGGGCAG CTCTGGCTAG 1381 ATGCCTATCT TCACCAATAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1441 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1501 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAATTATA TACCGAAGTC 1561 CCTCCCGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt