Construct: ORF TRCN0000468148
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015519.1_s317c1
- Derived from:
- ccsbBroadEn_15960
- DNA Barcode:
- CCATTTCTAAAACTCACCAAGATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CELA2A (63036)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468148
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 63036 | CELA2A | chymotrypsin like elastase 2A | NM_033440.3 | 99.6% | 99.6% | 17T>C;504T>C;672G>A |
| 2 | human | 51032 | CELA2B | chymotrypsin like elastase 2B | NM_015849.3 | 92.9% | 87.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 873
- ORF length:
- 807
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat aaggacgctg ccgctgtcca ctttggtggc tggagccctc agttgtgggg 121 accccactta cccaccttat gtgactaggg tggttggcgg tgaagaagcg aggcccaaca 181 gctggccctg gcaggtctcc ctgcagtaca gctccaatgg caagtggtac cacacctgcg 241 gagggtccct gatagccaac agctgggtcc tgacggctgc ccactgcatc agctcctcca 301 ggacctaccg cgtggggctg ggccggcaca acctctacgt tgcggagtcc ggctcgctgg 361 cagtcagtgt ctctaagatt gtggtgcaca aggactggaa ctccaaccaa atctccaaag 421 ggaacgacat tgccctgctc aaactggcta accccgtctc cctcaccgac aagatccagc 481 tggcctgcct ccctcctgcc ggcaccattc tacccaacaa ctacccctgc TACGTCACGG 541 GCTGGGGAAG GCTGCAGACC AACGGGGCCG TTCCTGATGT CCTGCAGCAG GGCCGGTTGC 601 TGGTTGTGGA CTATGCCACC TGCTCCAGCT CTGCCTGGTG GGGCAGCAGC GTGAAAACCA 661 GTATGATCTG TGCTGGGGGT GATGGCGTGA TCTCCAGCTG CAACGGAGAC TCTGGCGGGC 721 CACTGAACTG TCAGGCATCT GACGGCCGGT GGCAGGTGCA CGGCATCGTC AGCTTCGGGT 781 CTCGCCTCGG CTGCAACTAC TACCACAAGC CCTCCGTCTT CACGCGGGTC TCCAATTACA 841 TCGACTGGAT CAATTCGGTG ATTGCAAATA ACTACCCAAC TTTCTTGTAC AAAGTGGTTG 901 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 961 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 1021 ATTTCTAAAA CTCACCAAGA TGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1081 ttgtgaaaga tt