Transcript: Human NM_033440.3

Homo sapiens chymotrypsin like elastase 2A (CELA2A), mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
CELA2A (63036)
Length:
916
CDS:
23..832

Additional Resources:

NCBI RefSeq record:
NM_033440.3
NBCI Gene record:
CELA2A (63036)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_033440.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000046973 CTTACCCACCTTATGTGACTA pLKO.1 84 CDS 100% 4.950 6.930 N CELA2A n/a
2 TRCN0000372119 GGACTGGAACTCCAACCAAAT pLKO_005 349 CDS 100% 10.800 7.560 N CELA2A n/a
3 TRCN0000372120 CAAATCTCCAAAGGGAACGAC pLKO_005 365 CDS 100% 2.640 1.848 N CELA2A n/a
4 TRCN0000046975 CCTTATGTGACTAGGGTGGTT pLKO.1 92 CDS 100% 2.640 1.848 N CELA2A n/a
5 TRCN0000377790 TGTTCCTGATGTCCTGCAGCA pLKO_005 526 CDS 100% 2.160 1.512 N CELA2A n/a
6 TRCN0000046977 CCTCGGCTGCAACTACTACCA pLKO.1 742 CDS 100% 0.880 0.616 N CELA2A n/a
7 TRCN0000046976 GCAGTCAGTGTCTCTAAGATT pLKO.1 317 CDS 100% 5.625 3.375 N CELA2A n/a
8 TRCN0000046974 CGACTGGATCAATTCGGTGAT pLKO.1 799 CDS 100% 4.050 2.025 Y CELA2A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_033440.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03900 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_03900 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000468164 GGCAATACAATTCCCACCACTTAT pLX_317 42.6% 100% 100% V5 n/a
4 TRCN0000488240 GTGGAGTTATAACGGGCCCATCCC pLX_317 29.3% 100% 100% V5 (not translated due to prior stop codon) n/a
5 ccsbBroadEn_15960 pDONR223 0% 99.6% 99.6% None 17T>C;504T>C;672G>A n/a
6 ccsbBroad304_15960 pLX_304 0% 99.6% 99.6% V5 17T>C;504T>C;672G>A n/a
7 TRCN0000468148 CCATTTCTAAAACTCACCAAGATG pLX_317 42.6% 99.6% 99.6% V5 17T>C;504T>C;672G>A n/a
8 ccsbBroadEn_08200 pDONR223 100% 93.3% 89.2% None (many diffs) n/a
9 ccsbBroad304_08200 pLX_304 0% 93.3% 89.2% V5 (many diffs) n/a
10 TRCN0000478344 ACCAGACTGTGTGTCAACAGTCAA pLX_317 42.5% 93.3% 89.2% V5 (many diffs) n/a
11 ccsbBroadEn_03183 pDONR223 100% 93.1% 88.4% None (many diffs) n/a
12 ccsbBroad304_03183 pLX_304 0% 93.1% 88.4% V5 (many diffs) n/a
13 TRCN0000467785 ATGGGAATATGTGCTAGCGATCAC pLX_317 37.3% 93.1% 88.4% V5 (many diffs) n/a
14 ccsbBroadEn_15818 pDONR223 0% 93% 88.1% None (many diffs) n/a
15 ccsbBroad304_15818 pLX_304 0% 93% 88.1% V5 (many diffs) n/a
16 TRCN0000479873 TTAGTCTAGGTCCTCCACTGGCAT pLX_317 42.6% 93% 88.1% V5 (many diffs) n/a
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