Transcript: Human NM_015849.3

Homo sapiens chymotrypsin like elastase 2B (CELA2B), mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
CELA2B (51032)
Length:
915
CDS:
23..832

Additional Resources:

NCBI RefSeq record:
NM_015849.3
NBCI Gene record:
CELA2B (51032)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_015849.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000051362 CGCGCCTGATATGTCTAGGAT pLKO.1 88 CDS 100% 3.000 4.200 N CELA2B n/a
2 TRCN0000372066 TGATATGCACCTGCAACGGAG pLKO_005 645 CDS 100% 2.160 3.024 N CELA2B n/a
3 TRCN0000051358 CCTGATATGTCTAGGATGCTT pLKO.1 92 CDS 100% 3.000 2.400 N CELA2B n/a
4 TRCN0000051360 CACCGTGAAGACGAATATGAT pLKO.1 604 CDS 100% 5.625 3.938 N CELA2B n/a
5 TRCN0000051359 CCTTGGTTGCAACTACTACTA pLKO.1 742 CDS 100% 4.950 3.465 N CELA2B n/a
6 TRCN0000372065 AGCATAACCTCTACGTTGCAG pLKO_005 282 CDS 100% 2.640 1.848 N CELA2B n/a
7 TRCN0000051361 CGCGTGATGCTGGGCCAGCAT pLKO.1 266 CDS 100% 0.000 0.000 N CELA2B n/a
8 TRCN0000377801 CTACTACTACAAGCCCTCCAT pLKO_005 754 CDS 100% 2.640 1.584 N CELA2B n/a
9 TRCN0000046974 CGACTGGATCAATTCGGTGAT pLKO.1 799 CDS 100% 4.050 2.025 Y CELA2A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_015849.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03183 pDONR223 100% 99.8% 99.6% None 530A>G n/a
2 ccsbBroad304_03183 pLX_304 0% 99.8% 99.6% V5 530A>G n/a
3 TRCN0000467785 ATGGGAATATGTGCTAGCGATCAC pLX_317 37.3% 99.8% 99.6% V5 530A>G n/a
4 ccsbBroadEn_15818 pDONR223 0% 99.7% 99.2% None 530A>G;623T>C n/a
5 ccsbBroad304_15818 pLX_304 0% 99.7% 99.2% V5 530A>G;623T>C n/a
6 TRCN0000479873 TTAGTCTAGGTCCTCCACTGGCAT pLX_317 42.6% 99.7% 99.2% V5 530A>G;623T>C n/a
7 ccsbBroadEn_08200 pDONR223 100% 99.5% 98.8% None (many diffs) n/a
8 ccsbBroad304_08200 pLX_304 0% 99.5% 98.8% V5 (many diffs) n/a
9 TRCN0000478344 ACCAGACTGTGTGTCAACAGTCAA pLX_317 42.5% 99.5% 98.8% V5 (many diffs) n/a
10 ccsbBroadEn_03900 pDONR223 100% 93% 88.1% None (many diffs) n/a
11 ccsbBroad304_03900 pLX_304 0% 93% 88.1% V5 (many diffs) n/a
12 TRCN0000468164 GGCAATACAATTCCCACCACTTAT pLX_317 42.6% 93% 88.1% V5 (many diffs) n/a
13 TRCN0000488240 GTGGAGTTATAACGGGCCCATCCC pLX_317 29.3% 93% 88.1% V5 (not translated due to prior stop codon) (many diffs) n/a
14 ccsbBroadEn_15960 pDONR223 0% 92.9% 87.7% None (many diffs) n/a
15 ccsbBroad304_15960 pLX_304 0% 92.9% 87.7% V5 (many diffs) n/a
16 TRCN0000468148 CCATTTCTAAAACTCACCAAGATG pLX_317 42.6% 92.9% 87.7% V5 (many diffs) n/a
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