Construct: ORF TRCN0000468163
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003610.1_s317c1
- Derived from:
- ccsbBroadEn_02384
- DNA Barcode:
- CTCTGACGGGGTCTTTAGATCTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMNDC1 (10285)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468163
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10285 | SMNDC1 | survival motor neuron domai... | NM_005871.4 | 100% | 100% | |
| 2 | human | 10285 | SMNDC1 | survival motor neuron domai... | XM_005269382.3 | 83.1% | 83.1% | 0_1ins119;2_3delTG |
| 3 | mouse | 76479 | Smndc1 | survival motor neuron domai... | NM_172429.2 | 90% | 99.5% | (many diffs) |
| 4 | mouse | 76479 | Smndc1 | survival motor neuron domai... | XM_006527441.3 | 90% | 99.5% | (many diffs) |
| 5 | mouse | 76479 | Smndc1 | survival motor neuron domai... | XM_006527440.3 | 88.5% | 97.9% | (many diffs) |
| 6 | mouse | 76479 | Smndc1 | survival motor neuron domai... | XM_006527442.3 | 75.4% | 82.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 780
- ORF length:
- 714
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc agaggattta gcaaagcagc tggcaagcta caaagctcag ctccagcaag 121 ttgaagctgc attatctgga aatggagaaa atgaagattt gctaaaattg aagaaagatt 181 tacaagaagt tatagaacta accaaagacc ttctgtcaac tcaaccttct gagacgcttg 241 caagttcaga cagttttgct tctactcaac ctactcattc atggaaagta ggagacaagt 301 gtatggcagt ctggagtgaa gatggacagt gttatgaagc ggagattgag gagatagatg 361 aagaaaatgg caccgctgca atcaccttTG CTGGTTATGG CAATGCTGAA GTGACTCCAC 421 TGTTGAACCT CAAGCCTGTA GAAGAAGGAA GGAAGGCAAA GGAGGACAGT GGCAACAAAC 481 CCATGTCAAA AAAAGAAATG ATTGCCCAGC AGCGTGAATA TAAAAAGAAG AAAGCTTTGA 541 AAAAAGCTCA GAGAATAAAA GAACTTGAGC AGGAAAGAGA GGACCAGAAA GTGAAATGGC 601 AACAATTCAA CAACAGAGCC TATTCTAAAA ACAAAAAAGG CCAGGTAAAG AGGAGTATTT 661 TTGCTTCACC TGAGAGTGTG ACTGGTAAAG TTGGAGTAGG AACCTGTGGA ATTGCTGATA 721 AACCTATGAC ACAATATCAA GATACCTCTA AATACAATGT CAGGCATTTG ATGCCTCAAT 781 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 841 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 901 TTTATATATC TTGTGGAAAG GACGACTCTG ACGGGGTCTT TAGATCTCTA CGCGTTAAGT 961 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt