Construct: ORF TRCN0000468182
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004313.1_s317c1
- Derived from:
- ccsbBroadEn_01632
- DNA Barcode:
- TTCTGGAACAGGACCTATCTACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYPL1 (6856)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468182
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6856 | SYPL1 | synaptophysin like 1 | NM_182715.3 | 100% | 100% | |
| 2 | human | 6856 | SYPL1 | synaptophysin like 1 | NM_006754.4 | 93% | 93% | 1_54del |
| 3 | mouse | 19027 | Sypl | synaptophysin-like protein | NM_198710.3 | 84% | 83.1% | (many diffs) |
| 4 | mouse | 19027 | Sypl | synaptophysin-like protein | NM_013635.3 | 78.2% | 77.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 789
- ORF length:
- 723
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cggcttccag atcaacctca acccgctcaa ggagccactc ggcttcatca 121 aggtcctcga gtggattgct tctatctttg cttttgccac ctgtggaggt tttaagggcc 181 aaacagaaat tcaagtgaat tgtcctcctg cagttactga gaataaaact gttacagcta 241 cttttggtta tccattcagg ttgaatgagg catcatttca gccacctcca ggtgtaaaca 301 tatgtgatgt aaattggaaa gattacgtcc TCATAGGCGA TTACTCTTCT TCTGCACAAT 361 TCTATGTTAC CTTTGCAGTC TTTGTGTTCC TGTACTGCAT TGCTGCCCTT CTGCTTTATG 421 TTGGCTACAC GAGTCTGTAT CTGGATAGTC GTAAACTTCC TATGATAGAC TTTGTTGTTA 481 CACTTGTTGC CACTTTTTTG TGGTTGGTGA GCACTTCAGC CTGGGCTAAA GCTCTGACAG 541 ATATTAAAAT AGCTACTGGT CACAATATTA TTGATGAACT TCCGCCTTGT AAGAAGAAAG 601 CAGTACTGTG TTACTTTGGC TCTGTGACCA GTATGGGATC CCTAAATGTA TCTGTGATAT 661 TTGGCTTTCT AAATATGATA CTCTGGGGAG GAAATGCTTG GTTTGTGTAC AAGGAGACCA 721 GCCTACACAG TCCATCAAAT ACATCTGCCC CTCATAGCCA AGGAGGTATT CCACCTCCTA 781 CCGGAATATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 841 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 901 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATTCTGG AACAGGACCT ATCTACCTAC 961 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt