Construct: ORF TRCN0000468213
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010509.1_s317c1
- Derived from:
- ccsbBroadEn_04185
- DNA Barcode:
- AACTTCGCGGTGAGAGAAACTACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM134 (80194)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468213
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80194 | TMEM134 | transmembrane protein 134 | NM_001078650.3 | 100% | 100% | |
| 2 | human | 80194 | TMEM134 | transmembrane protein 134 | XM_006718693.2 | 95% | 95% | 236_237ins27 |
| 3 | human | 80194 | TMEM134 | transmembrane protein 134 | NM_025124.4 | 92.3% | 92.3% | 406_450del |
| 4 | human | 80194 | TMEM134 | transmembrane protein 134 | NM_001078651.3 | 87.6% | 87.6% | 236_237ins27;379_423del |
| 5 | human | 80194 | TMEM134 | transmembrane protein 134 | XM_017018356.1 | 77.6% | 73% | (many diffs) |
| 6 | human | 80194 | TMEM134 | transmembrane protein 134 | XM_011545268.3 | 77.1% | 75.5% | (many diffs) |
| 7 | human | 80194 | TMEM134 | transmembrane protein 134 | XM_024448697.1 | 73% | 33.3% | 0_1ins31;296_407del;487_531del |
| 8 | human | 80194 | TMEM134 | transmembrane protein 134 | XR_950062.3 | 60.3% | 1_80del;316_326del;632_895del | |
| 9 | human | 80194 | TMEM134 | transmembrane protein 134 | XR_001747983.2 | 48.7% | (many diffs) | |
| 10 | human | 80194 | TMEM134 | transmembrane protein 134 | XR_950065.1 | 47.5% | (many diffs) | |
| 11 | human | 80194 | TMEM134 | transmembrane protein 134 | NR_073412.2 | 47.1% | (many diffs) | |
| 12 | human | 80194 | TMEM134 | transmembrane protein 134 | XR_950061.1 | 46.8% | (many diffs) | |
| 13 | human | 80194 | TMEM134 | transmembrane protein 134 | XR_950064.3 | 33.7% | (many diffs) | |
| 14 | human | 80194 | TMEM134 | transmembrane protein 134 | NR_073411.2 | 15.1% | (many diffs) | |
| 15 | human | 80194 | TMEM134 | transmembrane protein 134 | NR_073410.2 | 14.8% | (many diffs) | |
| 16 | human | 80194 | TMEM134 | transmembrane protein 134 | NR_073413.2 | 14.6% | (many diffs) | |
| 17 | human | 80194 | TMEM134 | transmembrane protein 134 | NR_073409.2 | 14.5% | 1_59del;465_676del;812_3718del | |
| 18 | mouse | 66990 | Tmem134 | transmembrane protein 134 | NM_025889.2 | 90.7% | 93.8% | (many diffs) |
| 19 | mouse | 66990 | Tmem134 | transmembrane protein 134 | NM_001078649.1 | 83.7% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 609
- ORF length:
- 540
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcgccgcc cggccccagt tcagcattga tgatgccttc gagctgtccc 121 tggaggacgg gggccctggg cccgagtcca gcggggTCGC GCGCTTTGGG CCGCTGCACT 181 TCGAGCGTCG GGCCCGGTTC GAGGTGGCTG ACGAGGACAA GCAGTCCCGG CTGCGCTACC 241 AGAACCTGGA GAACGATGAG GATGGAGCCC AGGCCTCTCC GGAGCCGGAT GGGGGAGTCG 301 GCACCAGGGA TTCCAGCCGA ACTTCCATCC GCAGCTCCCA GTGGTCCTTC AGCACCATCA 361 GCAGCAGCAC CCAGCGCTCC TACAACACCT GCTGCAGCTG GACCCAACAC CCTTTGATCC 421 AGAAGAACCG CCGAGTGGTG CTGGCCTCCT TCCTGCTCCT GCTGCTGGGG CTGGGTGTCT 481 CCAGCGCCAT CTTCTTCGTG CCGGGCTTCC TGTTGTTGGT GCCTGGAGTC TATCACGTGA 541 TCTTCATCTA CTGCGCGGTC AAGGGCCACC GGGGCTTCCA GTTCTTCTAC CTGCCCTACT 601 TCGAGAAGTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAACTTC GCGGTGAGAG AAACTACGAC 781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt