Construct: ORF TRCN0000468216

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003175.1_s317c1
Derived from:
ccsbBroadEn_06640
DNA Barcode:
AGCCTCCGCCTCAAGGCTGACCAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NGF (4803)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468216
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4803 NGF nerve growth factor NM_002506.3 99.8% 99.5% 104C>T
2 human 4803 NGF nerve growth factor XM_006710663.3 99.8% 99.5% 104C>T
3 human 4803 NGF nerve growth factor XM_011541518.2 81.3% 81% 1_165del;269C>T
4 mouse 18049 Ngf nerve growth factor NM_001112698.2 85% 84.6% (many diffs)
5 mouse 18049 Ngf nerve growth factor NM_013609.3 66.8% 66.4% (many diffs)
6 mouse 18049 Ngf nerve growth factor XM_006501108.3 66.8% 66.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
792
ORF length:
723
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gtccatgttg ttctacactc tgatcacagc ttttctgatc ggcatacagg
121cggaaccaca ctcagagagc aatgtccctg caggacacac catcccccaa gtccactgga
181ctaaacttca gcattccctt gacactgccc ttcgcagagc ccgcagcgcc ccggcagcgg
241cgatagctgc acgcgtggcg gggcagaccc gcaacattac tgtggacccc aggctgttta
301aaaagcggcg actccgttca ccccgtgtgc tgtttagcac ccagcctccc cgtgaagctg
361cagacactca ggatctggac ttcGAGGTCG GTGGTGCTGC CCCCTTCAAC AGGACTCACA
421GGAGCAAGCG GTCATCATCC CATCCCATCT TCCACAGGGG CGAATTCTCG GTGTGTGACA
481GTGTCAGCGT GTGGGTTGGG GATAAGACCA CCGCCACAGA CATCAAGGGC AAGGAGGTGA
541TGGTGTTGGG AGAGGTGAAC ATTAACAACA GTGTATTCAA ACAGTACTTT TTTGAGACCA
601AGTGCCGGGA CCCAAATCCC GTTGACAGCG GGTGCCGGGG CATTGACTCA AAGCACTGGA
661ACTCATATTG TACCACGACT CACACCTTTG TCAAGGCGCT GACCATGGAT GGCAAGCAGG
721CTGCCTGGCG GTTTATCCGG ATAGATACGG CCTGTGTGTG TGTGCTCAGC AGGAAGGCTG
781TGAGAAGAGC CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
841ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
901CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAGC CTCCGCCTCA AGGCTGACCA
961CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)