Construct: ORF TRCN0000468216
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003175.1_s317c1
- Derived from:
- ccsbBroadEn_06640
- DNA Barcode:
- AGCCTCCGCCTCAAGGCTGACCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NGF (4803)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468216
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4803 | NGF | nerve growth factor | NM_002506.3 | 99.8% | 99.5% | 104C>T |
| 2 | human | 4803 | NGF | nerve growth factor | XM_006710663.3 | 99.8% | 99.5% | 104C>T |
| 3 | human | 4803 | NGF | nerve growth factor | XM_011541518.2 | 81.3% | 81% | 1_165del;269C>T |
| 4 | mouse | 18049 | Ngf | nerve growth factor | NM_001112698.2 | 85% | 84.6% | (many diffs) |
| 5 | mouse | 18049 | Ngf | nerve growth factor | NM_013609.3 | 66.8% | 66.4% | (many diffs) |
| 6 | mouse | 18049 | Ngf | nerve growth factor | XM_006501108.3 | 66.8% | 66.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 792
- ORF length:
- 723
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccatgttg ttctacactc tgatcacagc ttttctgatc ggcatacagg 121 cggaaccaca ctcagagagc aatgtccctg caggacacac catcccccaa gtccactgga 181 ctaaacttca gcattccctt gacactgccc ttcgcagagc ccgcagcgcc ccggcagcgg 241 cgatagctgc acgcgtggcg gggcagaccc gcaacattac tgtggacccc aggctgttta 301 aaaagcggcg actccgttca ccccgtgtgc tgtttagcac ccagcctccc cgtgaagctg 361 cagacactca ggatctggac ttcGAGGTCG GTGGTGCTGC CCCCTTCAAC AGGACTCACA 421 GGAGCAAGCG GTCATCATCC CATCCCATCT TCCACAGGGG CGAATTCTCG GTGTGTGACA 481 GTGTCAGCGT GTGGGTTGGG GATAAGACCA CCGCCACAGA CATCAAGGGC AAGGAGGTGA 541 TGGTGTTGGG AGAGGTGAAC ATTAACAACA GTGTATTCAA ACAGTACTTT TTTGAGACCA 601 AGTGCCGGGA CCCAAATCCC GTTGACAGCG GGTGCCGGGG CATTGACTCA AAGCACTGGA 661 ACTCATATTG TACCACGACT CACACCTTTG TCAAGGCGCT GACCATGGAT GGCAAGCAGG 721 CTGCCTGGCG GTTTATCCGG ATAGATACGG CCTGTGTGTG TGTGCTCAGC AGGAAGGCTG 781 TGAGAAGAGC CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 841 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 901 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAGC CTCCGCCTCA AGGCTGACCA 961 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t