Construct: ORF TRCN0000468229
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006264.1_s317c1
- Derived from:
- ccsbBroadEn_06947
- DNA Barcode:
- ACCCCATGAGAACTTCAGATCCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SHMT2 (6472)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468229
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | NM_005412.6 | 99.9% | 100% | 813G>A |
| 2 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | NM_001166356.2 | 97.9% | 98% | 594_595ins30;783G>A |
| 3 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | NM_001166357.1 | 95.7% | 95.8% | 0_1ins63;750G>A |
| 4 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | NM_001166358.2 | 95.7% | 95.8% | 0_1ins63;750G>A |
| 5 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | NM_001166359.1 | 95.7% | 95.8% | 0_1ins63;750G>A |
| 6 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | XM_011538675.3 | 77.5% | 62.4% | 0_1ins316;279_306del;525G>A |
| 7 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | XM_011538676.3 | 77.5% | 62.4% | 0_1ins316;279_306del;525G>A |
| 8 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | XM_011538677.3 | 77.5% | 62.4% | 0_1ins316;279_306del;525G>A |
| 9 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | XM_011538678.2 | 77.5% | 62.4% | 0_1ins316;279_306del;525G>A |
| 10 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | NR_029417.2 | 66.3% | (many diffs) | |
| 11 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | NR_029416.2 | 65.9% | (many diffs) | |
| 12 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | NR_029415.1 | 65.2% | (many diffs) | |
| 13 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | NR_048562.1 | 57.9% | (many diffs) | |
| 14 | mouse | 108037 | Shmt2 | serine hydroxymethyltransfe... | NM_028230.4 | 87.9% | 93.8% | (many diffs) |
| 15 | mouse | 108037 | Shmt2 | serine hydroxymethyltransfe... | NM_001252316.1 | 87.3% | 93.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1578
- ORF length:
- 1512
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gtacttctct ttgttttggg cggctcggcc tctgcagaga tgtgggcagc 121 tggtcaggat ggccattcgg gctcagcaca gcaacgcagc ccagactcag actggggaag 181 caaacagggg ctggacaggc caggagagcc tgtcggacag tgatcctgag atgtgggagt 241 tgctgcagag ggagaaggac aggcagtgtc gtggcctgga gctcattgcc tcagagaact 301 tctgcagccg agctgcgctg gaggccctgg ggtcctgtct gaacaacaag tactcggagg 361 gttatcctgg caagagatac tatgggggag cagaggtggt ggatgaaatt gagctgctgt 421 gccagcgccg ggccttggaa gcctttgacc tggatcctgc acagtgggga gtcaatgtcc 481 agccctactc cgggtcccca gccaacctgg ccgtctacac agcccttctg caacctcacg 541 accggatcat ggggctggac ctgcccgatg ggggccatct cacccacggc tacatgtctg 601 acgtcaagcg gatatcagcc acgtccatct tcttcgagtc tatgccctat aagctcaacc 661 ccaaaactgg cctcattgac tacaaccagc tggcactgac tgctcgactt ttccggccac 721 ggctcatcat agctggcacc agcgcctatg ctcgcctcat tgactacgcc cgcatgagag 781 aggtgtgtga tgaagtcaaa gcacacctgc tggcagacat ggcccacatc agtggcctgg 841 tggctgccaa ggtgattccc tcgcctttca agcacgcaga catcgtcacc accactactc 901 acaagactct tcgaggggcc aggtcagggc tcatcttcta ccggaaaggg gtgaaggctg 961 tggaccccaa gactggccgg gagatccctt acacatttga ggaccgaatc aactttgccg 1021 tgttcccatc cctgcagggg ggcccccaca atcatgccat tgctgcagta gctgtggccc 1081 taaagcaggc ctgcaccccc atgttccggg agtactccct gcaggttctg aagaatgctc 1141 gggccatggc agatgccctG CTAGAGCGAG GCTACTCACT GGTATCAGGT GGTACTGACA 1201 ACCACCTGGT GCTGGTGGAC CTGCGGCCCA AGGGCCTGGA TGGAGCTCGG GCTGAGCGGG 1261 TGCTAGAGCT TGTATCCATC ACTGCCAACA AGAACACCTG TCCTGGAGAC CGAAGTGCCA 1321 TCACACCGGG CGGCCTGCGG CTTGGGGCCC CAGCCTTAAC TTCTCGACAG TTCCGTGAGG 1381 ATGACTTCCG GAGAGTTGTG GACTTTATAG ATGAAGGGGT CAACATTGGC TTAGAGGTGA 1441 AGAGCAAGAC TGCCAAGCTC CAGGATTTCA AATCCTTCCT GCTTAAGGAC TCAGAAACAA 1501 GTCAGCGTCT GGCCAACCTC AGGCAACGGG TGGAGCAGTT TGCCAGGGCC TTCCCCATGC 1561 CTGGTTTTGA TGAGCATTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1621 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTTGAAAG TATTTCGATT 1681 TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACCCCATG AGAACTTCAG ATCCGGACGC 1741 GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt