Construct: ORF TRCN0000468242
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009676.1_s317c1
- Derived from:
- ccsbBroadEn_00022
- DNA Barcode:
- CAGTAAACTCTCTAACCCTGTTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACYP2 (98)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468242
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 98 | ACYP2 | acylphosphatase 2 | NM_138448.4 | 100% | 100% | |
| 2 | human | 98 | ACYP2 | acylphosphatase 2 | NM_001320588.2 | 75.7% | 75.7% | 0_1ins72 |
| 3 | human | 98 | ACYP2 | acylphosphatase 2 | NM_001320589.2 | 67.4% | 64.6% | (many diffs) |
| 4 | human | 98 | ACYP2 | acylphosphatase 2 | NM_001320590.2 | 65.3% | 62.6% | (many diffs) |
| 5 | human | 98 | ACYP2 | acylphosphatase 2 | NM_001320587.2 | 64.9% | 60.2% | (many diffs) |
| 6 | human | 98 | ACYP2 | acylphosphatase 2 | NM_001320586.2 | 49.3% | 46% | (many diffs) |
| 7 | human | 98 | ACYP2 | acylphosphatase 2 | XM_017005411.1 | 42.9% | 40% | (many diffs) |
| 8 | human | 98 | ACYP2 | acylphosphatase 2 | XM_017005410.1 | 40.9% | 28.7% | (many diffs) |
| 9 | human | 98 | ACYP2 | acylphosphatase 2 | XM_017005412.1 | 27.3% | 22.9% | (many diffs) |
| 10 | human | 98 | ACYP2 | acylphosphatase 2 | XM_017005413.1 | 25.2% | 22.1% | (many diffs) |
| 11 | human | 98 | ACYP2 | acylphosphatase 2 | XR_001739083.1 | 16.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 387
- ORF length:
- 297
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTCTACCGC CCAGTCACTC AAATCCGTGG 121 ACTACGAGGT GTTCGGAAGA GTGCAGGGTG TTTGCTTCAG AATGTATACA GAAGATGAAG 181 CTAGGAAAAT AGGAGTGGTT GGCTGGGTGA AGAATACCAG CAAAGGCACC GTGACAGGCC 241 AAGTGCAGGG GCCAGAAGAC AAAGTCAATT CCATGAAGTC CTGGCTGAGC AAGGTTGGAA 301 GCCCTAGTTC TCGCATTGAC CGCACAAACT TTTCTAATGA AAAAACCATC TCTAAGCTTG 361 AATACTCTAA TTTTAGTATT AGATACTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 421 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 481 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACAGTAAAC 541 TCTCTAACCC TGTTCCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 601 aagatt